The prenatal exposure to ethanol (Eth), fluconazole (FLUCO) and sodium valproate (VPA) is related to effects on development, producing characteristic syndromic pictures. Among embryotoxic effects described for the three molecules, the alteration on craniofacial morphogenesis is a common feature in humans and animal models, including rodent embryos developed in vitro. The aim of the present work is to evaluate the developmental effects of low Eth serum concentration (17 mM, corresponding to the legal limit to drive in UK, USA, Canada, and many other countries) in mixture with increasing realistic concentrations of the antifungal drug FLUCO (62.5–500 µM) or with increasing realistic concentrations of the antiepileptic drug VPA (31.25–250 µM). Groups exposed to Eth alone (17–127.5 mM), FLUCO alone (62.5–500 µM) or VPA alone (31.25–750 µM) were also included. The chosen alternative animal model was the post-implantation rat whole embryo culture (WEC). E9.5 embryos were exposed in vitro to the test molecules during the whole test period (48 h, corresponding to the developmental stages characteristics of any vertebrate, for human embryos post-fertilization days 23–31). Data were statistically analyzed and processed for modelling applying the benchmark dose (BMD) and relative potency factor (RPF) approaches. Concentration-related effects on facial outcomes were observed in all experimental groups, with a significant enhancement in the groups co-exposed with Eth in comparison to the single exposures. Data obtained by the present work suggest an additional alert for the assumption of even low levels of alcohol in pregnant women during FLUCO or VPA therapy.

Moderate alcohol consumption during pregnancy increases potency of two diferent drugs (the antifungal fuconazole and the antiepileptic valproate) in inducing craniofacial defects : prediction by the in vitro rat whole embryo culture / F. Metruccio, M. Battistoni, F. Di Renzo, A. Moretto, E. Menegola. - In: ARCHIVES OF TOXICOLOGY. - ISSN 0340-5761. - (2022 Nov 16). [Epub ahead of print] [10.1007/s00204-022-03410-2]

Moderate alcohol consumption during pregnancy increases potency of two diferent drugs (the antifungal fuconazole and the antiepileptic valproate) in inducing craniofacial defects : prediction by the in vitro rat whole embryo culture

F. Metruccio
Primo
;
M. Battistoni;F. Di Renzo
;
A. Moretto
Penultimo
;
E. Menegola
Ultimo
2022

Abstract

The prenatal exposure to ethanol (Eth), fluconazole (FLUCO) and sodium valproate (VPA) is related to effects on development, producing characteristic syndromic pictures. Among embryotoxic effects described for the three molecules, the alteration on craniofacial morphogenesis is a common feature in humans and animal models, including rodent embryos developed in vitro. The aim of the present work is to evaluate the developmental effects of low Eth serum concentration (17 mM, corresponding to the legal limit to drive in UK, USA, Canada, and many other countries) in mixture with increasing realistic concentrations of the antifungal drug FLUCO (62.5–500 µM) or with increasing realistic concentrations of the antiepileptic drug VPA (31.25–250 µM). Groups exposed to Eth alone (17–127.5 mM), FLUCO alone (62.5–500 µM) or VPA alone (31.25–750 µM) were also included. The chosen alternative animal model was the post-implantation rat whole embryo culture (WEC). E9.5 embryos were exposed in vitro to the test molecules during the whole test period (48 h, corresponding to the developmental stages characteristics of any vertebrate, for human embryos post-fertilization days 23–31). Data were statistically analyzed and processed for modelling applying the benchmark dose (BMD) and relative potency factor (RPF) approaches. Concentration-related effects on facial outcomes were observed in all experimental groups, with a significant enhancement in the groups co-exposed with Eth in comparison to the single exposures. Data obtained by the present work suggest an additional alert for the assumption of even low levels of alcohol in pregnant women during FLUCO or VPA therapy.
pregnancy; embryo; mixture; ethanol; antifungal; antiepileptic
Settore MED/42 - Igiene Generale e Applicata
Settore BIO/06 - Anatomia Comparata e Citologia
Settore BIO/14 - Farmacologia
H20_RIA15AMORE_M - Assessing the health risks of combined human exposure to multiple food-related toxic substances - MORETTO, ANGELO - H20_RIA - Horizon 2020_Research & Innovation Action/Innovation Action - 2015
16-nov-2022
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/946333
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