We have investigated the biological fluids--serum, cerebrospinal fluid, and urine--of three strains of rats; the present data extend our database (also available on-line) and may be of interest for pharmacological and toxicological investigation. Specifically, we have defined reference maps of the major protein components in cerebrospinal fluid and urine. Compartment-specific isoforms were recognized for transferrin and transthyretin. Mass spectrometric data established the cleavage site of the signal peptide and identified the N-terminal blocking group of prostaglandin D synthase from rat cerebrospinal fluid. A previously undescribed member of the family of low molecular mass rat urinary proteins was characterized as containing a sequence similar, but not identical, to the N-terminal region of rat urinary protein-2 (RUP-2), and divergent from RUP-1.

Proteins of rat serum, urine, and cerebrospinal fluid : VI. Further protein identifications and interstrain comparison / R. Wait, E. Gianazza, I. Eberini, L. Sironi, M.J. Dunn, M. Gemeiner, I. Miller. - In: ELECTROPHORESIS. - ISSN 0173-0835. - 22:14(2001 Aug), pp. 3043-3052.

Proteins of rat serum, urine, and cerebrospinal fluid : VI. Further protein identifications and interstrain comparison

E. Gianazza
Secondo
;
I. Eberini;L. Sironi;
2001

Abstract

We have investigated the biological fluids--serum, cerebrospinal fluid, and urine--of three strains of rats; the present data extend our database (also available on-line) and may be of interest for pharmacological and toxicological investigation. Specifically, we have defined reference maps of the major protein components in cerebrospinal fluid and urine. Compartment-specific isoforms were recognized for transferrin and transthyretin. Mass spectrometric data established the cleavage site of the signal peptide and identified the N-terminal blocking group of prostaglandin D synthase from rat cerebrospinal fluid. A previously undescribed member of the family of low molecular mass rat urinary proteins was characterized as containing a sequence similar, but not identical, to the N-terminal region of rat urinary protein-2 (RUP-2), and divergent from RUP-1.
rat ; serum ; urine ; cerebrospinal fluid ; two-dimensional electrophoresis
Settore BIO/10 - Biochimica
ago-2001
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/9129
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