The immune system is a complex and finely orchestrated system, and many soluble molecules and receptors contribute to its regulation. Recent studies have suggested that many of the modulatory effects induced by morphine on innate immunity, and in particular the effects on macrophage activation and function, can be due to the modulation of an important macrophage surface receptor, the toll-like receptor (TLR), that is primarily involved in early regulatory steps. In this chapter we describe a RT-real-time PCR method for assessing TLR expression in macrophage after in vivo morphine treatment.

Measurement of Macrophage Toll-Like Receptor 4 Expression After Morphine Treatment / M. Castelli, A.E. Panerai, P.G. Sacerdote, S. Franchi (METHODS IN MOLECULAR BIOLOGY). - In: Opioid Receptors : Methods and Protocols . / [a cura di] S.M. Spampinato. - [s.l] : Springer, 2021. - ISBN 9781071608838. - pp. 209-217

Measurement of Macrophage Toll-Like Receptor 4 Expression After Morphine Treatment

M. Castelli;A.E. Panerai;P.G. Sacerdote;S. Franchi
2021

Abstract

The immune system is a complex and finely orchestrated system, and many soluble molecules and receptors contribute to its regulation. Recent studies have suggested that many of the modulatory effects induced by morphine on innate immunity, and in particular the effects on macrophage activation and function, can be due to the modulation of an important macrophage surface receptor, the toll-like receptor (TLR), that is primarily involved in early regulatory steps. In this chapter we describe a RT-real-time PCR method for assessing TLR expression in macrophage after in vivo morphine treatment.
Macrophage; Morphine; Opioid receptor; RT-real-time; PCR; TLR4
Settore BIO/14 - Farmacologia
2021
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/767688
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