Streptomyces exfoliatus” FT05W is a biocontrol strain showedantagonisms to a variety of soil-borne pathogens. In this study, weused Miseq PE300 next-generation sequencing platform to per-form its genome sequencing, and obtained 6914188 reads. TheSPAdes software was used to splice a total of 1836 contigs witha total genome length of 7699129 bp and a GC ratio of 71%, inwhich the length of the coding gene sequence is 6037181 bp, and7434 protein-coding genes are predicted, which are predicted toencode 7233 proteins. Subsequently, through the comparison andanalysis of databases such as GO, COG, KEGG, NR and TCDB, thegenome prediction and basic function annotations were completed.The gene annotation information thus obtained lays an importantfoundation for further research on its biological control mechanismin plant. The basis of the study will help promote the excavationand utilization of “Streptomyces” sp. functional genes and provide atheoretical basis for further development and application of “Strep-tomyces exfoliatus” FT05W as biopesticides.

Draft genome sequencing of Streptomyces exfoliatus FT05W, a biological control strain against plant soil-borne pathogens / X. Chen, K. Qin, W. Sang, M. Saracchi, P. Cortesi. - In: JOURNAL OF BIOTECHNOLOGY. - ISSN 0168-1656. - 395:Supplement(2019 Nov 15), pp. S70-S70. ((Intervento presentato al convegno European Biotechnology Congress: April, 11th - 13th tenutosi a Valencia (Spain) nel 2019 [10.1016/j.jbiotec.2019.05.245].

Draft genome sequencing of Streptomyces exfoliatus FT05W, a biological control strain against plant soil-borne pathogens

X. Chen
Primo
;
M. Saracchi
Penultimo
;
P. Cortesi
Ultimo
2019

Abstract

Streptomyces exfoliatus” FT05W is a biocontrol strain showedantagonisms to a variety of soil-borne pathogens. In this study, weused Miseq PE300 next-generation sequencing platform to per-form its genome sequencing, and obtained 6914188 reads. TheSPAdes software was used to splice a total of 1836 contigs witha total genome length of 7699129 bp and a GC ratio of 71%, inwhich the length of the coding gene sequence is 6037181 bp, and7434 protein-coding genes are predicted, which are predicted toencode 7233 proteins. Subsequently, through the comparison andanalysis of databases such as GO, COG, KEGG, NR and TCDB, thegenome prediction and basic function annotations were completed.The gene annotation information thus obtained lays an importantfoundation for further research on its biological control mechanismin plant. The basis of the study will help promote the excavationand utilization of “Streptomyces” sp. functional genes and provide atheoretical basis for further development and application of “Strep-tomyces exfoliatus” FT05W as biopesticides.
Settore AGR/12 - Patologia Vegetale
15-nov-2019
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/751014
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