To determine whether hyperhomocysteinemia induced post-methionine loading (PML) is associated with different response in the aminothiol redox state and oxidative stress vs. normohomocysteinemia, we assessed PML plasma thiols, vitamins, free malondialdehyde (MDA), and blood reduced glutathione (GSH) in 120 consecutive subjects (50 [35-56] years, 83 males), divided into two groups according to PML plasma total Hcy < 35 microM (Group 1, n = 65) or > or = 35 microM (Group 2, n = 55). In the group as a whole, plasma reduced cysteine and cysteinylglycine, blood reduced GSH (all p for time = 0.0001) and plasma total GSH (p for time = 0.001) increased from baseline to PML. MDA values were unchanged. Group 1 and 2 differed in blood reduced GSH (p for group = 0.004, higher in Group 2), and MDA levels (p for group = 0.024, lower in Group 2). The oxidative stress induced by methionine challenge seems to be opposed by scavenger molecules activation, namely GSH, and lipid peroxidation does not increase. This mechanism paradoxically appears to be more efficient in hyperhomocysteinemic subjects

Methionine challenge paradoxically induces a greater activation of the antioxidant defence in subjects with hyper- vs normohomocysteinemia / J. Campolo, B. De Chiara, R. Caruso, R. De Maria, V. Sedda, C. Dellanoce, M. Parolini, G. Cighetti, S. Penco, F. Baudo, O. Parodi. - In: FREE RADICAL RESEARCH. - ISSN 1071-5762. - 40:9(2006 Sep), pp. 929-935.

Methionine challenge paradoxically induces a greater activation of the antioxidant defence in subjects with hyper- vs normohomocysteinemia

B. De Chiara;V. Sedda;G. Cighetti;
2006

Abstract

To determine whether hyperhomocysteinemia induced post-methionine loading (PML) is associated with different response in the aminothiol redox state and oxidative stress vs. normohomocysteinemia, we assessed PML plasma thiols, vitamins, free malondialdehyde (MDA), and blood reduced glutathione (GSH) in 120 consecutive subjects (50 [35-56] years, 83 males), divided into two groups according to PML plasma total Hcy < 35 microM (Group 1, n = 65) or > or = 35 microM (Group 2, n = 55). In the group as a whole, plasma reduced cysteine and cysteinylglycine, blood reduced GSH (all p for time = 0.0001) and plasma total GSH (p for time = 0.001) increased from baseline to PML. MDA values were unchanged. Group 1 and 2 differed in blood reduced GSH (p for group = 0.004, higher in Group 2), and MDA levels (p for group = 0.024, lower in Group 2). The oxidative stress induced by methionine challenge seems to be opposed by scavenger molecules activation, namely GSH, and lipid peroxidation does not increase. This mechanism paradoxically appears to be more efficient in hyperhomocysteinemic subjects
Aminothiols; Antioxidants; Glutathione; Homocysteine; Methionine loading; Oxidative stress
Settore BIO/10 - Biochimica
set-2006
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/73304
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