In the last decade, it has become evident that RNA is frequently found in DNA. It is now well established that single embedded ribonucleoside monophosphates (rNMPs) are primarily introduced by DNA polymerases and that longer stretches of RNA can anneal to DNA, generating RNA:DNA hybrids. Among them, the most studied are R‐loops, peculiar three‐stranded nucleic acid structures formed upon the re‐hybridization of a transcript to its template DNA. In addition, polyribonucleotide chains are synthesized to allow DNA replication priming, double‐strand breaks repair, and may as well result from the direct incorporation of consecutive rNMPs by DNA polymerases. The bright side of RNA into DNA is that it contributes to regulating different physiological functions. The dark side, however, is that persistent RNA compromises genome integrity and genome stability. For these reasons, the characterization of all these structures has been under growing investigation. In this review, we discussed the origin of single and multiple ribonucleotides in the genome and in the DNA of organelles, focusing on situations where the aberrant processing of RNA:DNA hybrids may result in multiple rNMPs embedded in DNA. We concluded by providing an overview of the currently available strategies to study the presence of single and multiple ribonucleotides in DNA in vivo.

One, no one, and one hundred thousand: The many forms of ribonucleotides in DNA / G.M. Nava, L. Grasso, S. Sertic, A. Pellicioli, M. Muzi Falconi, F. Lazzaro. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1661-6596. - 21:5(2020), pp. 1706.1-1706.23. [10.3390/ijms21051706]

One, no one, and one hundred thousand: The many forms of ribonucleotides in DNA

G.M. Nava;L. Grasso;S. Sertic;A. Pellicioli;M. Muzi Falconi;F. Lazzaro
2020

Abstract

In the last decade, it has become evident that RNA is frequently found in DNA. It is now well established that single embedded ribonucleoside monophosphates (rNMPs) are primarily introduced by DNA polymerases and that longer stretches of RNA can anneal to DNA, generating RNA:DNA hybrids. Among them, the most studied are R‐loops, peculiar three‐stranded nucleic acid structures formed upon the re‐hybridization of a transcript to its template DNA. In addition, polyribonucleotide chains are synthesized to allow DNA replication priming, double‐strand breaks repair, and may as well result from the direct incorporation of consecutive rNMPs by DNA polymerases. The bright side of RNA into DNA is that it contributes to regulating different physiological functions. The dark side, however, is that persistent RNA compromises genome integrity and genome stability. For these reasons, the characterization of all these structures has been under growing investigation. In this review, we discussed the origin of single and multiple ribonucleotides in the genome and in the DNA of organelles, focusing on situations where the aberrant processing of RNA:DNA hybrids may result in multiple rNMPs embedded in DNA. We concluded by providing an overview of the currently available strategies to study the presence of single and multiple ribonucleotides in DNA in vivo.
No
English
rNMPs incorporation; RNA:DNA hybrids; RNase H; replication stress; genome instability
Settore BIO/11 - Biologia Molecolare
Review essay
Esperti anonimi
Ricerca di base
Pubblicazione scientifica
2020
MDPI AG
21
5
1706
1
23
23
Pubblicato
Periodico con rilevanza internazionale
scopus
orcid
pubmed
crossref
Aderisco
info:eu-repo/semantics/article
One, no one, and one hundred thousand: The many forms of ribonucleotides in DNA / G.M. Nava, L. Grasso, S. Sertic, A. Pellicioli, M. Muzi Falconi, F. Lazzaro. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1661-6596. - 21:5(2020), pp. 1706.1-1706.23. [10.3390/ijms21051706]
open
Prodotti della ricerca::01 - Articolo su periodico
6
262
Article (author)
no
G.M. Nava, L. Grasso, S. Sertic, A. Pellicioli, M. Muzi Falconi, F. Lazzaro
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/727388
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