Human tryptase is a serine protease expressed in mast-cells. We previously observed that AML blast cells, cultured in vitro from a KIT D816Y patient, give rise to adherent cells with mast-cell like phenotype and tryptase was released in the serum-free medium. To correlate total serum tryptase (ts-try) levels with cytogenetic features and KIT mutational status, we analyzed serum samples from AML patients at diagnosis. In 70 out of 155 patients (45%) we detected elevated ts-try (>15 ng/mL), significantly linked to t(8;21) (P < .001) and inv(16) (P = .007). In patients that achieved complete remission the ts-try decreased to normal values. In 75 patients screened for KIT mutation, we found a clear relationship between elevated ts-try and mutated patients with t(8;21) (P < .001). In conclusion, we propose that checking for ts-try at diagnosis of AML may be a simple tool to select patients to be addressed to KIT mutation screening.
Total serum tryptase : a predictive marker for KIT mutation in acute myeloid leukemia / R. Cairoli, C.B. Ripamonti, A. Beghini, S. Granata, G. Grillo, M. Brioschi, G. Nadali, A. Viola, C. Cattaneo, L. Inropido, E. Ravelli, G. Bertani, L. Pezzetti, M. Nichelatti, A. Marocchi, G. Rossi, G. Pizzolo, F. Ferrara, A.M. Nosari, E. Morra. - In: LEUKEMIA RESEARCH. - ISSN 0145-2126. - 33:9(2009), pp. 1282-1284.
Total serum tryptase : a predictive marker for KIT mutation in acute myeloid leukemia
A. Beghini;
2009
Abstract
Human tryptase is a serine protease expressed in mast-cells. We previously observed that AML blast cells, cultured in vitro from a KIT D816Y patient, give rise to adherent cells with mast-cell like phenotype and tryptase was released in the serum-free medium. To correlate total serum tryptase (ts-try) levels with cytogenetic features and KIT mutational status, we analyzed serum samples from AML patients at diagnosis. In 70 out of 155 patients (45%) we detected elevated ts-try (>15 ng/mL), significantly linked to t(8;21) (P < .001) and inv(16) (P = .007). In patients that achieved complete remission the ts-try decreased to normal values. In 75 patients screened for KIT mutation, we found a clear relationship between elevated ts-try and mutated patients with t(8;21) (P < .001). In conclusion, we propose that checking for ts-try at diagnosis of AML may be a simple tool to select patients to be addressed to KIT mutation screening.
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