In the present project, financed by Fondazione Cariplo, cheese whey permeate, the residual by-product obtained by extraction of whey proteins from cheese whey, is used as substrate for the growth of bacterial species that produce the anti-listerial bacteriocin sakacin-A from Lactobacillus sakei and bacterial cellulose from Komagataeibacter xylinus. BC is then turned into nanocrystals that are finally conjugated with sakacin-A to obtain an innovative antimicrobial device for food which could support Listeria monocytogenes growth. Antimicrobial materials were prepared from sakacin A and/or conjugates applying incorporation, grafting or coating techniques, comparatively. An economic analysis of cheese whey market at Europe level was also carried out as well as the estimation of the production cost of the most performing material developed, even if at preliminary lab-scale level. Antimicrobial activity of the obtained materials against Listeria was finally confirmed either by in vitro and in vivo trials on real food products, demonstrating that the developed device can contribute to increase shelf life and quality of perishable foods, thus decreasing the loss of food resources.

Conjugates of bacterial cellulose nanocrystals and sakacin-A in an Anti-Listeria food packaging material / M. Rollini, A. Musatti, C. Mapelli, P. Motta, S. Farris, D. Romano, R. Gavara, P. Hernandez, L. Higueras, D. Bussini, D. Cavicchioli, D. Bertoni. ((Intervento presentato al convegno MATBIM tenutosi a Milano nel 2019.

Conjugates of bacterial cellulose nanocrystals and sakacin-A in an Anti-Listeria food packaging material

M. Rollini
;
A. Musatti;C. Mapelli;P. Motta;S. Farris;D. Romano;D. Cavicchioli;D. Bertoni
2019

Abstract

In the present project, financed by Fondazione Cariplo, cheese whey permeate, the residual by-product obtained by extraction of whey proteins from cheese whey, is used as substrate for the growth of bacterial species that produce the anti-listerial bacteriocin sakacin-A from Lactobacillus sakei and bacterial cellulose from Komagataeibacter xylinus. BC is then turned into nanocrystals that are finally conjugated with sakacin-A to obtain an innovative antimicrobial device for food which could support Listeria monocytogenes growth. Antimicrobial materials were prepared from sakacin A and/or conjugates applying incorporation, grafting or coating techniques, comparatively. An economic analysis of cheese whey market at Europe level was also carried out as well as the estimation of the production cost of the most performing material developed, even if at preliminary lab-scale level. Antimicrobial activity of the obtained materials against Listeria was finally confirmed either by in vitro and in vivo trials on real food products, demonstrating that the developed device can contribute to increase shelf life and quality of perishable foods, thus decreasing the loss of food resources.
8-mag-2019
sakacin A, active packaging, nanocellulose
Settore AGR/16 - Microbiologia Agraria
Settore AGR/15 - Scienze e Tecnologie Alimentari
Conjugates of bacterial cellulose nanocrystals and sakacin-A in an Anti-Listeria food packaging material / M. Rollini, A. Musatti, C. Mapelli, P. Motta, S. Farris, D. Romano, R. Gavara, P. Hernandez, L. Higueras, D. Bussini, D. Cavicchioli, D. Bertoni. ((Intervento presentato al convegno MATBIM tenutosi a Milano nel 2019.
Conference Object
File in questo prodotto:
File Dimensione Formato  
MATBIM2019-Final-program.pdf

accesso aperto

Tipologia: Altro
Dimensione 300.67 kB
Formato Adobe PDF
300.67 kB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/665632
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact