Polycomb repressive complexes are evolutionarily conserved complexes that maintain transcriptional repression during development and differentiation to establish and preserve cell identity. We recently described the fundamental role of PRC1 in preserving intestinal stem cell identity through the inhibition of non–lineage-specific transcription factors. To further elucidate the role of PRC1 in adult stem cell maintenance, we now investigated its role in LGR5 + hair follicle stem cells during regeneration. We show that PRC1 depletion severely affects hair regeneration and, different from intestinal stem cells, derepression of its targets induces the ectopic activation of an epidermal-specific program. Our data support a general role of PRC1 in preserving stem cell identity that is shared between different compartments. However, the final outcome of the ectopic activation of non–lineage-specific transcription factors observed upon loss of PRC1 is largely context-dependent and likely related to the transcription factors repertoire and specific epigenetic landscape of different cellular compartments.

Loss of PRC1 activity in different stem cell compartments activates a common transcriptional program with cell type–dependent outcomes / S. Pivetti, D. Fernandez-Perez, A. D'Ambrosio, C.M. Barbieri, D. Manganaro, A. Rossi, L. Barnabei, M. Zanotti, A. Scelfo, F. Chiacchiera, D. Pasini. - In: SCIENCE ADVANCES. - ISSN 2375-2548. - 5:5(2019), pp. eaav1594.1-eaav1594.11. [10.1126/sciadv.aav1594]

Loss of PRC1 activity in different stem cell compartments activates a common transcriptional program with cell type–dependent outcomes

S. Pivetti
Primo
;
D. Fernandez-Perez
Secondo
;
A. D'Ambrosio;M. Zanotti;A. Scelfo;D. Pasini
Ultimo
2019

Abstract

Polycomb repressive complexes are evolutionarily conserved complexes that maintain transcriptional repression during development and differentiation to establish and preserve cell identity. We recently described the fundamental role of PRC1 in preserving intestinal stem cell identity through the inhibition of non–lineage-specific transcription factors. To further elucidate the role of PRC1 in adult stem cell maintenance, we now investigated its role in LGR5 + hair follicle stem cells during regeneration. We show that PRC1 depletion severely affects hair regeneration and, different from intestinal stem cells, derepression of its targets induces the ectopic activation of an epidermal-specific program. Our data support a general role of PRC1 in preserving stem cell identity that is shared between different compartments. However, the final outcome of the ectopic activation of non–lineage-specific transcription factors observed upon loss of PRC1 is largely context-dependent and likely related to the transcription factors repertoire and specific epigenetic landscape of different cellular compartments.
English
Polycomb group proteins; comprehensive guide; roles; classification; progenitors; expression; complexes; intestine; subunits; identity
Settore BIO/11 - Biologia Molecolare
Articolo
Esperti anonimi
Ricerca di base
Pubblicazione scientifica
2019
American Association for the Advancement of Science
5
5
eaav1594
1
11
11
Pubblicato
Periodico con rilevanza internazionale
Aderisco
info:eu-repo/semantics/article
Loss of PRC1 activity in different stem cell compartments activates a common transcriptional program with cell type–dependent outcomes / S. Pivetti, D. Fernandez-Perez, A. D'Ambrosio, C.M. Barbieri, D. Manganaro, A. Rossi, L. Barnabei, M. Zanotti, A. Scelfo, F. Chiacchiera, D. Pasini. - In: SCIENCE ADVANCES. - ISSN 2375-2548. - 5:5(2019), pp. eaav1594.1-eaav1594.11. [10.1126/sciadv.aav1594]
open
Prodotti della ricerca::01 - Articolo su periodico
11
262
Article (author)
no
S. Pivetti, D. Fernandez-Perez, A. D'Ambrosio, C.M. Barbieri, D. Manganaro, A. Rossi, L. Barnabei, M. Zanotti, A. Scelfo, F. Chiacchiera, D. Pasini...espandi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/661508
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