Mycobacterium avium has become a major human pathogen, primarily due to the emergence of the;AIDS epidemic. Restriction fragment length polymorphism (RFLP) typing, using insertion sequence IS1245 as a probe, provides a powerful tool in the molecular epidemiology of M. avium-related infections and will facilitate well-founded studies into the sources of M. avium infections in animal and environmental reservoirs. The standardization of this technique allows computerization of IS1245 RFLP patterns for comparison on a local level and the establishment of M. avium DNA fingerprint databases for interlaboratory comparison. Moreover, by combining international DNA typing results of M. avium complex isolates from a broad spectrum of sources, long-lasting questions on the epidemiology of this major agent of mycobacterial infections will be answered.
IS1245 restriction fragment length polymorphism typing of Mycobacterium avium isolates: Proposal for standardization / D. Van Soolingen, J. Bauer, V. Ritacco, S. Leao, I. Pavlik, V. Vincent, N. Rastogi, A. Gori, T. Bodmer, C. Garzelli, M. Garcia. - In: JOURNAL OF CLINICAL MICROBIOLOGY. - ISSN 0095-1137. - 36:10(1998), pp. 3051-3054.
IS1245 restriction fragment length polymorphism typing of Mycobacterium avium isolates: Proposal for standardization
A. Gori;
1998
Abstract
Mycobacterium avium has become a major human pathogen, primarily due to the emergence of the;AIDS epidemic. Restriction fragment length polymorphism (RFLP) typing, using insertion sequence IS1245 as a probe, provides a powerful tool in the molecular epidemiology of M. avium-related infections and will facilitate well-founded studies into the sources of M. avium infections in animal and environmental reservoirs. The standardization of this technique allows computerization of IS1245 RFLP patterns for comparison on a local level and the establishment of M. avium DNA fingerprint databases for interlaboratory comparison. Moreover, by combining international DNA typing results of M. avium complex isolates from a broad spectrum of sources, long-lasting questions on the epidemiology of this major agent of mycobacterial infections will be answered.File | Dimensione | Formato | |
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