MicroRNAs are small (19-24 nt) noncoding RNAs that play an important role in the regulation of multiple cell events, inhibiting gene expression at the posttranscriptional level by binding target mRNAs that are subsequently degraded or sequestered from translation. The availability of the complete genome sequence of the grapevine (Vitis vinifera), has already permitted genome-wide predictions of microRNAs by purely computational methods. Here we integrate transcriptomic data derived from high-throughput Illumina SOLEXA and ABI SOLiD sequence tags derived from both polyA+ transcripts and isolated small RNAs with oligonucleotide array data. We are thus able to detect both mature microRNAs and to establish whether genomic loci corresponding to the pre-miRNA are expressed in various tissues. In many cases, the unambiguous alignment of sequence tags derived from polyA+ RNA to the genomic sequence allow provisional mapping of primary microRNA transcripts, a first step towards bioinformatics characterization of elements potentially regulating microRNA expression.

Characterization and analysis of the expression pattern of microRNAs in the grapevine Vitis vinifera / V. Piccolo, E. Mica, E. Pè, G. Pesole, D. Horner. ((Intervento presentato al 10. convegno FISV Annual Congress tenutosi a Riva del Garda nel 2008.

Characterization and analysis of the expression pattern of microRNAs in the grapevine Vitis vinifera

V. Piccolo
Primo
;
E. Mica
Secondo
;
E. Pè;G. Pesole
Penultimo
;
D. Horner
Ultimo
2008

Abstract

MicroRNAs are small (19-24 nt) noncoding RNAs that play an important role in the regulation of multiple cell events, inhibiting gene expression at the posttranscriptional level by binding target mRNAs that are subsequently degraded or sequestered from translation. The availability of the complete genome sequence of the grapevine (Vitis vinifera), has already permitted genome-wide predictions of microRNAs by purely computational methods. Here we integrate transcriptomic data derived from high-throughput Illumina SOLEXA and ABI SOLiD sequence tags derived from both polyA+ transcripts and isolated small RNAs with oligonucleotide array data. We are thus able to detect both mature microRNAs and to establish whether genomic loci corresponding to the pre-miRNA are expressed in various tissues. In many cases, the unambiguous alignment of sequence tags derived from polyA+ RNA to the genomic sequence allow provisional mapping of primary microRNA transcripts, a first step towards bioinformatics characterization of elements potentially regulating microRNA expression.
2008
Federazione Italiana Scienze della Vita
Characterization and analysis of the expression pattern of microRNAs in the grapevine Vitis vinifera / V. Piccolo, E. Mica, E. Pè, G. Pesole, D. Horner. ((Intervento presentato al 10. convegno FISV Annual Congress tenutosi a Riva del Garda nel 2008.
Conference Object
File in questo prodotto:
File Dimensione Formato  
fisv2008.pdf

accesso aperto

Tipologia: Post-print, accepted manuscript ecc. (versione accettata dall'editore)
Dimensione 18.39 kB
Formato Adobe PDF
18.39 kB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/62783
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact