Fidelity of chromosome segregation is ensured by a tension-dependent error correction system that prevents stabilization of incorrect chromosome-microtubule attachments. Unattached or incorrectly attached chromosomes also activate the spindle assembly checkpoint, thus delaying mitotic exit until all chromosomes are bioriented. The Aurora B kinase is widely recognized as a component of error correction. Conversely, its role in the checkpoint is controversial. Here, we report an analysis of the role of Aurora B in the spindle checkpoint under conditions believed to uncouple the effects of Aurora B inhibition on the checkpoint from those on error correction. Partial inhibition of several checkpoint and kinetochore components, including Mps1 and Ndc80, strongly synergizes with inhibition of Aurora B activity and dramatically affects the ability of cells to arrest in mitosis in the presence of spindle poisons. Thus, Aurora B might contribute to spindle checkpoint signalling independently of error correction. Our results support a model in which Aurora B is at the apex of a signalling pyramid whose sensory apparatus promotes the concomitant activation of error correction and checkpoint signalling pathways. The EMBO Journal (2011) 30, 1508-1519. doi:10.1038/emboj.2011.70; Published online 15 March 2011 Subject Categories: cell cycle

Evidence that Aurora B is implicated in spindle checkpoint signalling independently of error correction / S. Santaguida, C. Vernieri, F. Villa, A. Ciliberto, A. Musacchio, F. RI villa. - In: EMBO JOURNAL. - ISSN 0261-4189. - 30:8(2011), pp. 1508-1519. [10.1038/emboj.2011.70]

Evidence that Aurora B is implicated in spindle checkpoint signalling independently of error correction

S. Santaguida
Primo
;
2011

Abstract

Fidelity of chromosome segregation is ensured by a tension-dependent error correction system that prevents stabilization of incorrect chromosome-microtubule attachments. Unattached or incorrectly attached chromosomes also activate the spindle assembly checkpoint, thus delaying mitotic exit until all chromosomes are bioriented. The Aurora B kinase is widely recognized as a component of error correction. Conversely, its role in the checkpoint is controversial. Here, we report an analysis of the role of Aurora B in the spindle checkpoint under conditions believed to uncouple the effects of Aurora B inhibition on the checkpoint from those on error correction. Partial inhibition of several checkpoint and kinetochore components, including Mps1 and Ndc80, strongly synergizes with inhibition of Aurora B activity and dramatically affects the ability of cells to arrest in mitosis in the presence of spindle poisons. Thus, Aurora B might contribute to spindle checkpoint signalling independently of error correction. Our results support a model in which Aurora B is at the apex of a signalling pyramid whose sensory apparatus promotes the concomitant activation of error correction and checkpoint signalling pathways. The EMBO Journal (2011) 30, 1508-1519. doi:10.1038/emboj.2011.70; Published online 15 March 2011 Subject Categories: cell cycle
centromere; hesperadin; kinetochore; reversine; tension
Settore BIO/11 - Biologia Molecolare
2011
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/623002
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