MTT Cell Proliferation Assay was used to optimize the concentration of Telomerase Restrictors(TRs) with minimum toxicity to the selected cells. FACSort flow cytometer and Innotest P24 HIV(Human immunodeficiency Virus) antigen mAb ELISA Kit were used to investigate the anti-HIV-1 activities of TRs. The results showed that TRs had low cytotoxicity to the PBMC (Peripheral Blood mononuclear cells) and CEM/GFP if the concentration of TRs was at 50 μmol/L or below, and the supernatant from PBMC pretreated with SHIV and TR1-001 /TR1-002 could not infect the PBMC, while can infect the C8166 with reduced infectivity, which suggested that the TRs may be one of the novel resources for screening anti-HIV-1 agents.

Anti-HIV-1 activities of 4 Telomerase Restrictors / X. Yu, J. Wang, C. De Giuli Morghen, A. Radaelli, C. Zanotto, P. Beggio. - In: WUHAN UNIVERSITY JOURNAL OF NATURAL SCIENCES. - ISSN 1007-1202. - 12:6(2007 Nov), pp. 1113-1117. [10.1007/s11859-007-0090-0]

Anti-HIV-1 activities of 4 Telomerase Restrictors

C. De Giuli Morghen;A. Radaelli;C. Zanotto
Penultimo
;
P. Beggio
Ultimo
2007

Abstract

MTT Cell Proliferation Assay was used to optimize the concentration of Telomerase Restrictors(TRs) with minimum toxicity to the selected cells. FACSort flow cytometer and Innotest P24 HIV(Human immunodeficiency Virus) antigen mAb ELISA Kit were used to investigate the anti-HIV-1 activities of TRs. The results showed that TRs had low cytotoxicity to the PBMC (Peripheral Blood mononuclear cells) and CEM/GFP if the concentration of TRs was at 50 μmol/L or below, and the supernatant from PBMC pretreated with SHIV and TR1-001 /TR1-002 could not infect the PBMC, while can infect the C8166 with reduced infectivity, which suggested that the TRs may be one of the novel resources for screening anti-HIV-1 agents.
telomerase ; telomerase restrictor ; HIV ; anti-HIV Agents Screening
Settore BIO/19 - Microbiologia Generale
nov-2007
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/62198
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