Mesoangioblasts (MABS) are mesoderm-derived stem cells, associated with small vessels and originally described in the mouse embryonic dorsal aorta. Similar though not identical cells have been later identified and characterized from postnatal small vessels of skeletal muscle and heart. They have in common the expression of pericyte markers, the anatomical location, the ability to self-renew in culture, and to differentiate into various types of mesodermal lineages upon proper culture conditions. Currently, the developmental origin of MABs and the relationship with other muscle stem cells are not understood in detail and are the subject of active research. This chapter provides an outline of the latest techniques for isolation and characterization of adult MABs from human and mouse skeletal muscles.

Mouse and human mesoangioblasts: isolation and characterization from adult skeletal muscles / M. Quattrocelli, G. Palazzolo, I. Perini, S. Crippa, M. Cassano, M. Sampaolesi (METHODS IN MOLECULAR BIOLOGY). - In: Myogenesis : Methods and Protocols / [a cura di] J.X. DiMario. - [s.l] : Springer, 2012 Oct. - ISBN 9781617793424. - pp. 65-76 [10.1007/978-1-61779-343-1_4]

Mouse and human mesoangioblasts: isolation and characterization from adult skeletal muscles

M. Cassano;
2012

Abstract

Mesoangioblasts (MABS) are mesoderm-derived stem cells, associated with small vessels and originally described in the mouse embryonic dorsal aorta. Similar though not identical cells have been later identified and characterized from postnatal small vessels of skeletal muscle and heart. They have in common the expression of pericyte markers, the anatomical location, the ability to self-renew in culture, and to differentiate into various types of mesodermal lineages upon proper culture conditions. Currently, the developmental origin of MABs and the relationship with other muscle stem cells are not understood in detail and are the subject of active research. This chapter provides an outline of the latest techniques for isolation and characterization of adult MABs from human and mouse skeletal muscles.
Mesoangioblasts; Pericytes; Muscle stem cells; FACS; Surface antigens; Cocultures; Mesodermal lineages
Settore BIO/17 - Istologia
ott-2012
Book Part (author)
File in questo prodotto:
File Dimensione Formato  
fulltext(1).pdf

accesso riservato

Tipologia: Publisher's version/PDF
Dimensione 850.05 kB
Formato Adobe PDF
850.05 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/618845
Citazioni
  • ???jsp.display-item.citation.pmc??? 19
  • Scopus 39
  • ???jsp.display-item.citation.isi??? 37
social impact