Oncolytic viruses (OV) are engineered to infect, replicate in and kill cancer cells. Currently, the OV therapeutic approach is mainly restricted to neoplasia amenable to direct local administration of viral particles, while the possibility of a systemic delivery of cancer-tropic viruses would extend the OV application to the treatment of metastatic neoplasia. Herein, we applied in vivo/ex vivo imaging to demonstrate that cancer tropism is achieved when OV are encapsulated inside extracellular vesicles (EV) administered intravenously (i.v.), but not when injected intraperitoneally (i.p.). Moreover, we show that the therapeutic procedure adopted does not alter the immunomodulatory properties of the viruses.

Systemic Administration and Targeted Delivery of Immunogenic Oncolytic Adenovirus Encapsulated in Extracellular Vesicles for Cancer Therapies / M. Garofalo, A. Villa, N. Rizzi, L. Kuryk, V. Mazzaferro, P. Ciana. - In: VIRUSES. - ISSN 1999-4915. - 10:10(2018 Oct 13).

Systemic Administration and Targeted Delivery of Immunogenic Oncolytic Adenovirus Encapsulated in Extracellular Vesicles for Cancer Therapies

M. Garofalo
Primo
;
A. Villa
Secondo
;
N. Rizzi;V. Mazzaferro
Penultimo
;
P. Ciana
Ultimo
2018

Abstract

Oncolytic viruses (OV) are engineered to infect, replicate in and kill cancer cells. Currently, the OV therapeutic approach is mainly restricted to neoplasia amenable to direct local administration of viral particles, while the possibility of a systemic delivery of cancer-tropic viruses would extend the OV application to the treatment of metastatic neoplasia. Herein, we applied in vivo/ex vivo imaging to demonstrate that cancer tropism is achieved when OV are encapsulated inside extracellular vesicles (EV) administered intravenously (i.v.), but not when injected intraperitoneally (i.p.). Moreover, we show that the therapeutic procedure adopted does not alter the immunomodulatory properties of the viruses.
Oncolytic adenovirus; extracellular vesicles; immunocompetent mouse model; in vivo imaging; lung cancer; Adenoviridae; Animals; Extracellular Vesicles; Humans; Lung Neoplasms; Mice; Mice, Inbred C57BL; Oncolytic Viruses; Oncolytic Virotherapy
Settore BIO/14 - Farmacologia
   Dipartimenti di Eccellenza 2018-2022 - Dipartimento di ONCOLOGIA ED EMATO-ONCOLOGIA
   MINISTERO DELL'ISTRUZIONE E DEL MERITO
13-ott-2018
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/611500
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