The genome of Helicobacter pylori encodes for carbonic anhydrases (CAs, EC 4.2.1.1) belonging to the aand b-CA classes, which together with urease, have a pivotal role in the acid acclimation of the microorganism within the human stomach. Recently, in the exoproteome of H. pylori, a CA with no indication of the corresponding class was identified. Here, using the protonography and the mass spectrometry, a CA belonging to the a-class was detected in the outer membrane vesicles (OMVs) generated by planktonic and biofilm phenotypes of four H. pylori strains. The amount of this metalloenzyme was higher in the planktonic OMVs (pOMVs) than in the biofilm OMVs (bOMVs). Furthermore, the content of a-CA increases over time in the pOMVs. The identification of the a-CA in pOMVs and bOMVs might shed new light on the role of this enzyme in the colonization, survival, persistence, and pathogenesis of H. pylori

Identification and characterization of the a-CA in the outer membrane vesicles produced by Helicobacter pylori / M. Roncia, S. Del Prete, V. Puca, S. Carradori, V. Carginale, R. Muraro, G. Mincione, A. Aceto, F. Sisto, C.T. Supuran, R. Grande, C. Capasso. - In: JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY. - ISSN 1475-6366. - 34:1(2019 Jan 01), pp. 189-195. [10.1080/14756366.2018.1539716]

Identification and characterization of the a-CA in the outer membrane vesicles produced by Helicobacter pylori

F. Sisto;
2019

Abstract

The genome of Helicobacter pylori encodes for carbonic anhydrases (CAs, EC 4.2.1.1) belonging to the aand b-CA classes, which together with urease, have a pivotal role in the acid acclimation of the microorganism within the human stomach. Recently, in the exoproteome of H. pylori, a CA with no indication of the corresponding class was identified. Here, using the protonography and the mass spectrometry, a CA belonging to the a-class was detected in the outer membrane vesicles (OMVs) generated by planktonic and biofilm phenotypes of four H. pylori strains. The amount of this metalloenzyme was higher in the planktonic OMVs (pOMVs) than in the biofilm OMVs (bOMVs). Furthermore, the content of a-CA increases over time in the pOMVs. The identification of the a-CA in pOMVs and bOMVs might shed new light on the role of this enzyme in the colonization, survival, persistence, and pathogenesis of H. pylori
No
English
Carbonic anydrases; Helicobacter pylori; outer membrane vesicles (OMVs); biofilm; protonography; mass spectrometry
Settore MED/07 - Microbiologia e Microbiologia Clinica
Articolo
Esperti anonimi
Ricerca di base
Pubblicazione scientifica
1-gen-2019
Taylor & Francis
34
1
189
195
7
Pubblicato
Periodico con rilevanza internazionale
Aderisco
info:eu-repo/semantics/article
Identification and characterization of the a-CA in the outer membrane vesicles produced by Helicobacter pylori / M. Roncia, S. Del Prete, V. Puca, S. Carradori, V. Carginale, R. Muraro, G. Mincione, A. Aceto, F. Sisto, C.T. Supuran, R. Grande, C. Capasso. - In: JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY. - ISSN 1475-6366. - 34:1(2019 Jan 01), pp. 189-195. [10.1080/14756366.2018.1539716]
open
Prodotti della ricerca::01 - Articolo su periodico
12
262
Article (author)
no
M. Roncia, S. Del Prete, V. Puca, S. Carradori, V. Carginale, R. Muraro, G. Mincione, A. Aceto, F. Sisto, C.T. Supuran, R. Grande, C. Capasso
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/609588
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