The aim of this study was to investigate whether microscopic methods and computer image analysis are useful in distinguish and classify marine/fish meals containing pelagic and farmed fish (e.g. salmon) vs sea mammals (no target species). Accordingly, eight samples of controlled origin were used, namely: fishmeal (4 samples) and sea mammal meals (4 reference samples). Specifically, two fish meals were from pelagic catch (FM Latin America and FM Scandinavia), one was Mackerel meal and one Salmon meal. Sea mammal meals (i.e no target species) were obtained from Dolphin, Porpoise, Seal or Whale carcass provided by the Walloon Agricultural Research Centre - CRA-W, Belgium, Europen Reference Laboratory for Animal Proteins. Samples were analyzed by the microscopic method, according to Annex VI of Regulation 152/2009. Sediment fractions of each sample were observed with a compound microscope at X40. Bone fragment lacunae (n. 625) images from 8 samples (FM Latin America, FM Scandinavia, Mackerel, Salmon, Dolphin, Porpoise, Seal or Whale) were recorded and processed through an IA software. Accordingly, on each lacunae 30 geometric variables have been obtained and measured. The geometric variables have been grouped in two main families, namely: size descriptors and derived shape descriptors. Considering the size descriptors, 11 of them have shown higher mean values in pelagic and farmed fish samples (FMs, mackerel and salmon) than in sea mammal pure meals. By contrast axis minor, diameter min, radius min, size width, ferret min were lower in other fish than in sea mammals. Of note, some differences within the group of fish (FM Latin America, FM Scandinavia, mackerel and salmon) have been also observed: three size descriptors referred to lacunae area were higher in salmon lacunae than in the other fish meals. With regard to the shape descriptor, with the exception of perimeter ratio and solidity, all variables were diverse in fish and sea mammal. Therefore, it can be concluded that combining microscopy and image analysis can contribute in distinguishing fish material vs marine mammal’s materials. Among fish, salmon have shown different bone lacunae size features.
Microscopic methods and computer image analysis for distinguishing fish meals containing pelagic and farmed fish vs sea mammals (no target species) / M. Ottoboni, M. Tretola, L. Pinotti - In: Feed 2018Prima edizione. - [s.l] : Institute of Marine Research Bergen, 2018 Oct. - pp. 59-59 (( Intervento presentato al 6. convegno International Feed Conference: Present and Future Challenges tenutosi a Bergen nel 2018.
Microscopic methods and computer image analysis for distinguishing fish meals containing pelagic and farmed fish vs sea mammals (no target species)
M. OttoboniPrimo
;M. TretolaSecondo
;L. Pinotti
2018
Abstract
The aim of this study was to investigate whether microscopic methods and computer image analysis are useful in distinguish and classify marine/fish meals containing pelagic and farmed fish (e.g. salmon) vs sea mammals (no target species). Accordingly, eight samples of controlled origin were used, namely: fishmeal (4 samples) and sea mammal meals (4 reference samples). Specifically, two fish meals were from pelagic catch (FM Latin America and FM Scandinavia), one was Mackerel meal and one Salmon meal. Sea mammal meals (i.e no target species) were obtained from Dolphin, Porpoise, Seal or Whale carcass provided by the Walloon Agricultural Research Centre - CRA-W, Belgium, Europen Reference Laboratory for Animal Proteins. Samples were analyzed by the microscopic method, according to Annex VI of Regulation 152/2009. Sediment fractions of each sample were observed with a compound microscope at X40. Bone fragment lacunae (n. 625) images from 8 samples (FM Latin America, FM Scandinavia, Mackerel, Salmon, Dolphin, Porpoise, Seal or Whale) were recorded and processed through an IA software. Accordingly, on each lacunae 30 geometric variables have been obtained and measured. The geometric variables have been grouped in two main families, namely: size descriptors and derived shape descriptors. Considering the size descriptors, 11 of them have shown higher mean values in pelagic and farmed fish samples (FMs, mackerel and salmon) than in sea mammal pure meals. By contrast axis minor, diameter min, radius min, size width, ferret min were lower in other fish than in sea mammals. Of note, some differences within the group of fish (FM Latin America, FM Scandinavia, mackerel and salmon) have been also observed: three size descriptors referred to lacunae area were higher in salmon lacunae than in the other fish meals. With regard to the shape descriptor, with the exception of perimeter ratio and solidity, all variables were diverse in fish and sea mammal. Therefore, it can be concluded that combining microscopy and image analysis can contribute in distinguishing fish material vs marine mammal’s materials. Among fish, salmon have shown different bone lacunae size features.File | Dimensione | Formato | |
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