Vascular endothelial growth factor (VEGF) is important for maintaining healthy endothelium, which is crucial for vascular integrity. In this paper, we show that VEGF stimulates the nuclear translocation of endothelial differentiation-related factor 1 (EDF1), a highly conserved intracellular protein implicated in molecular events that are pivotal to endothelial function. In the nucleus, EDF1 serves as a transcriptional coactivator of peroxisome proliferator-activated receptor gamma (PPAR gamma), which has a protective role in the vasculature. Indeed, silencing EDF1 prevents VEGF induction of PPAR gamma activity as detected by gene reporter assay. Accordingly, silencing EDF1 markedly inhibits the stimulatory effect of VEGF on the expression of FABP4, a PPAR gamma-inducible gene. As nitric oxide is a marker of endothelial function, it is noteworthy that we report a link between EDF1 silencing, decreased levels of FABP4, and nitric oxide production. We conclude that EDF1 is required for VEGF-induced activation of the transcriptional activity of PPAR gamma

The contribution of EDF1 to PPARγ transcriptional activation in VEGF-treated human endothelial cells / A. Cazzaniga, L. Locatelli, S. Castiglioni, J. Maier. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1661-6596. - 19:7(2018 Jul). [10.3390/ijms19071830]

The contribution of EDF1 to PPARγ transcriptional activation in VEGF-treated human endothelial cells

A. Cazzaniga
Primo
Membro del Collaboration Group
;
L. Locatelli
Secondo
Membro del Collaboration Group
;
S. Castiglioni
Penultimo
Membro del Collaboration Group
;
J. Maier
Ultimo
Conceptualization
2018

Abstract

Vascular endothelial growth factor (VEGF) is important for maintaining healthy endothelium, which is crucial for vascular integrity. In this paper, we show that VEGF stimulates the nuclear translocation of endothelial differentiation-related factor 1 (EDF1), a highly conserved intracellular protein implicated in molecular events that are pivotal to endothelial function. In the nucleus, EDF1 serves as a transcriptional coactivator of peroxisome proliferator-activated receptor gamma (PPAR gamma), which has a protective role in the vasculature. Indeed, silencing EDF1 prevents VEGF induction of PPAR gamma activity as detected by gene reporter assay. Accordingly, silencing EDF1 markedly inhibits the stimulatory effect of VEGF on the expression of FABP4, a PPAR gamma-inducible gene. As nitric oxide is a marker of endothelial function, it is noteworthy that we report a link between EDF1 silencing, decreased levels of FABP4, and nitric oxide production. We conclude that EDF1 is required for VEGF-induced activation of the transcriptional activity of PPAR gamma
endothelial cells; vascular endothelial growth factor; Peroxisome proliferator-activated receptor gamma; Endothelial Differentiation-related factor 1
Settore MED/04 - Patologia Generale
lug-2018
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/589781
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