Polyamidoaminoacids (PAACs) are a new class of stimuli-responsive bioinspired chiral polymers. Recently, amphoteric PAACs isomers named ARGO7 were obtained by the polyaddition of L-, D- and D,L-arginine to an aqueous solution of N,N′-methylenebisacrylamide.[1] Molecular modeling studies in water showed that L- and D-ARGO7 folded into rigid and compact conformations driven by the polymer main chain flexibility. In order to demonstrate that the observed behavior was a general one, a library of amphoteric PAACs were synthesized from different L-, D- and D,L α-aminoacids by stepwise polyaddition to N,N'-methylenebisacrylamide. The reaction occurred in water at pH > 10 and 50 °C for 6 days with 92% yield, Mn from 4000 – 6000 and PD 1.40. Hydrodynamic radius (Rh) was determined by Dynamic Light Scattering (DLS), revealing only one family of nanoparticles with 1.5 nm average radius. CD spectra, recorded in the 3-12 pH interval, were consistent with the presence of pH-dependent ordered secondary structures, whose changes with pH were rapid and fully reversible. The dependence on temperature, ionic strength and presence of denaturating agents was assessed for the hydrophobic α-aminoacids deriving PAACs. CD spectral pattern were insensitive to all these parameters, suggesting highly stable and rigid structures. Future studies on cell internalization will assess the potential of PAACs for establishing chirality-dependent selective interactions with cell components.
Insight into the self-assembly behavior of nanostructured amino acid-deriving polymers / F. Lazzari, A.G. Manfredi, J. Alongi, E. Ranucci, P. Ferruti. ((Intervento presentato al convegno Science and Engineering of Polymeric Materials tenutosi a Sousse, nel 2018.
Insight into the self-assembly behavior of nanostructured amino acid-deriving polymers
F. LazzariPrimo
;A.G. Manfredi;J. Alongi;E. Ranucci;P. Ferruti
2018
Abstract
Polyamidoaminoacids (PAACs) are a new class of stimuli-responsive bioinspired chiral polymers. Recently, amphoteric PAACs isomers named ARGO7 were obtained by the polyaddition of L-, D- and D,L-arginine to an aqueous solution of N,N′-methylenebisacrylamide.[1] Molecular modeling studies in water showed that L- and D-ARGO7 folded into rigid and compact conformations driven by the polymer main chain flexibility. In order to demonstrate that the observed behavior was a general one, a library of amphoteric PAACs were synthesized from different L-, D- and D,L α-aminoacids by stepwise polyaddition to N,N'-methylenebisacrylamide. The reaction occurred in water at pH > 10 and 50 °C for 6 days with 92% yield, Mn from 4000 – 6000 and PD 1.40. Hydrodynamic radius (Rh) was determined by Dynamic Light Scattering (DLS), revealing only one family of nanoparticles with 1.5 nm average radius. CD spectra, recorded in the 3-12 pH interval, were consistent with the presence of pH-dependent ordered secondary structures, whose changes with pH were rapid and fully reversible. The dependence on temperature, ionic strength and presence of denaturating agents was assessed for the hydrophobic α-aminoacids deriving PAACs. CD spectral pattern were insensitive to all these parameters, suggesting highly stable and rigid structures. Future studies on cell internalization will assess the potential of PAACs for establishing chirality-dependent selective interactions with cell components.File | Dimensione | Formato | |
---|---|---|---|
Poster Lazzari Federica_Tunisia.pdf
accesso aperto
Descrizione: Poster
Tipologia:
Publisher's version/PDF
Dimensione
301.39 kB
Formato
Adobe PDF
|
301.39 kB | Adobe PDF | Visualizza/Apri |
Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.