Our study investigates the effect of iron deficiency on morpho-physiological and biochemical parameters of two Medicago ciliaris ecotypes (Mateur TN11.11 and Soliman TN8.7). Iron deficiency was imposed by making plants grow, either in an iron free or by the addition of CaCO 3/NaHCO3 to the Hoagland nutrient solution. Our results showed that both true and bicarbonate Fe-deficiency induced the characteristic iron-chlorosis symptoms, although the intensity of the symptoms was ecotype-dependent. This variability in tolerance to iron deficiency was also displayed by other morphological parameters such as root biomass and chlorophyll concentration. Besides, iron chlorosis induced an increase in biochemical parameters: the iron reducing capacity (measured in vivo on root segments and in vitro on plasma membrane enriched vesicles) and rhizosphere acidification by enhancement of H+-ATPase activity were more pronounced in Mateur ecotype. These findings suggest that Soliman ecotype was more sensitive than Mateur one to iron chlorosis.

Responses of two ecotypes of Medicago ciliaris to direct and bicarbonate-induced iron deficiency conditions / W. M’sehli, M. Dell’Orto, P. De Nisi, S. Donnini, C. Abdelly, G. Zocchi, M. Gharsalli. - In: ACTA PHYSIOLOGIAE PLANTARUM. - ISSN 0137-5881. - 31:4(2009 Jul), pp. 667-673.

Responses of two ecotypes of Medicago ciliaris to direct and bicarbonate-induced iron deficiency conditions

M. Dell’Orto
Secondo
;
P. De Nisi;S. Donnini;G. Zocchi
Penultimo
;
2009

Abstract

Our study investigates the effect of iron deficiency on morpho-physiological and biochemical parameters of two Medicago ciliaris ecotypes (Mateur TN11.11 and Soliman TN8.7). Iron deficiency was imposed by making plants grow, either in an iron free or by the addition of CaCO 3/NaHCO3 to the Hoagland nutrient solution. Our results showed that both true and bicarbonate Fe-deficiency induced the characteristic iron-chlorosis symptoms, although the intensity of the symptoms was ecotype-dependent. This variability in tolerance to iron deficiency was also displayed by other morphological parameters such as root biomass and chlorophyll concentration. Besides, iron chlorosis induced an increase in biochemical parameters: the iron reducing capacity (measured in vivo on root segments and in vitro on plasma membrane enriched vesicles) and rhizosphere acidification by enhancement of H+-ATPase activity were more pronounced in Mateur ecotype. These findings suggest that Soliman ecotype was more sensitive than Mateur one to iron chlorosis.
Fe(III)-chelate reductase; H+-ATPase; Iron deficiency; Medicago ciliaris; Variability
Settore AGR/13 - Chimica Agraria
lug-2009
Article (author)
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/57784
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 24
  • ???jsp.display-item.citation.isi??? 19
social impact