Eukaryotic cells plasma membranes are organized into microdomains of specialized function such as lipid rafts and caveolae, with a specific lipid composition highly enriched in cholesterol and glycosphingolipids. In addition to their role in regulating signal transduction, multiple functions have been proposed, such as anchorage of receptors, trafficking of cholesterol, and regulation of permeability. However, an extensive understanding of their protein composition in human heart, both in failing and non-failing conditions, is not yet available. Membrane microdomains were isolated from left ventricular tissue of both failing (n = 15) and non-failing (n = 15) human hearts. Protein composition and differential protein expression was explored by comparing series of 2-D maps and subsequent identification by LC-MS/MS analysis. Data indicated that heart membrane microdomains are enriched in chaperones, cytoskeletal-associated proteins, enzymes and protein involved in signal transduction pathway. In addition, differential protein expression profile revealed that 30 proteins were specifically up- or down-regulated in human heart failure membrane microdomains. This study resulted in the identification of human heart membrane microdomain protein composition, which was not previously available. Moreover, it allowed the identification of multiple proteins whose expression is altered in heart failure, thus opening new perspectives to determine which role they may play in this disease.

Proteomic analysis of membrane microdomains derived from both failing and non-failing human hearts / C. Banfi, M. Brioschi, R. Wait, S. Begum, E. Gianazza, P. Fratto, G. Polvani, E. Vitali, A. Parolari, L. Mussoni, E. Tremoli. - In: PROTEOMICS. - ISSN 1615-9853. - 6:6(2006), pp. 1976-1988. [10.1002/pmic.200500278]

Proteomic analysis of membrane microdomains derived from both failing and non-failing human hearts

C. Banfi
;
M. Brioschi
Secondo
;
E. Gianazza;G. Polvani;A. Parolari;L. Mussoni
Penultimo
;
E. Tremoli
Ultimo
2006

Abstract

Eukaryotic cells plasma membranes are organized into microdomains of specialized function such as lipid rafts and caveolae, with a specific lipid composition highly enriched in cholesterol and glycosphingolipids. In addition to their role in regulating signal transduction, multiple functions have been proposed, such as anchorage of receptors, trafficking of cholesterol, and regulation of permeability. However, an extensive understanding of their protein composition in human heart, both in failing and non-failing conditions, is not yet available. Membrane microdomains were isolated from left ventricular tissue of both failing (n = 15) and non-failing (n = 15) human hearts. Protein composition and differential protein expression was explored by comparing series of 2-D maps and subsequent identification by LC-MS/MS analysis. Data indicated that heart membrane microdomains are enriched in chaperones, cytoskeletal-associated proteins, enzymes and protein involved in signal transduction pathway. In addition, differential protein expression profile revealed that 30 proteins were specifically up- or down-regulated in human heart failure membrane microdomains. This study resulted in the identification of human heart membrane microdomain protein composition, which was not previously available. Moreover, it allowed the identification of multiple proteins whose expression is altered in heart failure, thus opening new perspectives to determine which role they may play in this disease.
2-DE; Heart proteins; Lipid rafts; Membrane subproteome; Proteome maps
Settore BIO/10 - Biochimica
Settore MED/23 - Chirurgia Cardiaca
Settore BIO/14 - Farmacologia
2006
Article (author)
File in questo prodotto:
File Dimensione Formato  
Banfi_et_al-2006-PROTEOMICS.pdf

accesso riservato

Tipologia: Publisher's version/PDF
Dimensione 316.49 kB
Formato Adobe PDF
316.49 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/5370
Citazioni
  • ???jsp.display-item.citation.pmc??? 14
  • Scopus 42
  • ???jsp.display-item.citation.isi??? 40
social impact