A collaborative exercise on DNA/RNA co-analysis and RNA cell typing involving 15 GEFI (Italian working group of ISFG) laboratories was organized in collaboration with the Netherlands Forensic Institute. Participants received: 1) PCR primers for a 19-plex mRNA profiling assay, with reference purified PCR products for each cell type targeted in the multiplex; 2) detailed protocols for DNA/RNA co-extraction, mRNA profiling, and interpretation of results; 3) a set of 8 mock forensic stains (7 single source, one a mixture of two body fluids). All but one laboratory generated correct DNA typing results. As expected, stochastic effects were seen for low template DNA extracted from a skin stain. As for mRNA profiling, the percentage of laboratories that correctly identified body fluids was ≥60% for blood, saliva, vaginal mucosa, semen, and skin. Success rates were <50% for menstrual secretions, nasal mucosa, and the mixed stain (menstrual secretions and saliva).
A GEFI collaborative exercise on DNA/RNA co-analysis and mRNA profiling interpretation / E. Carnevali, D. Lacerenza, S. Severini, F. Alessandrini, C. Bini, C. Di Nunzio, M. Di Nunzio, M. Fabbri, P. Fattorini, A. Piccinini, E. Ponzano, G. Portera, C. Previderè, A. Renieri, F. Scarnicci, A. Verzeletti, S. Pelotti, M. van den Berged, T. Sijen, C. Robino. - In: FORENSIC SCIENCE INTERNATIONAL: GENETICS SUPPLEMENT SERIES. - ISSN 1875-1768. - 6(2017 Dec), pp. e18-e20. ((Intervento presentato al 27. convegno Congress of the International Society for Forensic Genetics (ISFG) tenutosi a Seoul nel 2017 [10.1016/j.fsigss.2017.09.018].
A GEFI collaborative exercise on DNA/RNA co-analysis and mRNA profiling interpretation
A. Piccinini;G. Portera;
2017
Abstract
A collaborative exercise on DNA/RNA co-analysis and RNA cell typing involving 15 GEFI (Italian working group of ISFG) laboratories was organized in collaboration with the Netherlands Forensic Institute. Participants received: 1) PCR primers for a 19-plex mRNA profiling assay, with reference purified PCR products for each cell type targeted in the multiplex; 2) detailed protocols for DNA/RNA co-extraction, mRNA profiling, and interpretation of results; 3) a set of 8 mock forensic stains (7 single source, one a mixture of two body fluids). All but one laboratory generated correct DNA typing results. As expected, stochastic effects were seen for low template DNA extracted from a skin stain. As for mRNA profiling, the percentage of laboratories that correctly identified body fluids was ≥60% for blood, saliva, vaginal mucosa, semen, and skin. Success rates were <50% for menstrual secretions, nasal mucosa, and the mixed stain (menstrual secretions and saliva).File | Dimensione | Formato | |
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