Highly purified pituitary bovine prolactin (bPRL) has been used in a sensitive non-competitive enzyme-linked immunosorbent assay of prolactin (PRL) concentrations in plasma. In this assay affinity purified polyclonal antibodies were immobilzed to the solid phase in order to capture the antigen, and were also biotinylated as the detector antibody. The method was found to be reproducible (3% variability between calibration curves) and has been optimized for measuring bPRL concentration in plasma samples, giving an intra-assay coefficient of variation of about 5% and an interassay coefficient of variation of about 9%. The sensitivity of the assay was found to be as low as 0.1 ng/ml of bPRL.

An enzyme-linked immunosorbent assay for the determination of bovine prolactin in plasma / C. Secchi, A. Berrini, V. Borromeo. - In: JOURNAL OF IMMUNOLOGICAL METHODS. - ISSN 0022-1759. - 136:1(1991), pp. 17-22.

An enzyme-linked immunosorbent assay for the determination of bovine prolactin in plasma

C. Secchi
;
A. Berrini
Secondo
;
V. Borromeo
Ultimo
1991

Abstract

Highly purified pituitary bovine prolactin (bPRL) has been used in a sensitive non-competitive enzyme-linked immunosorbent assay of prolactin (PRL) concentrations in plasma. In this assay affinity purified polyclonal antibodies were immobilzed to the solid phase in order to capture the antigen, and were also biotinylated as the detector antibody. The method was found to be reproducible (3% variability between calibration curves) and has been optimized for measuring bPRL concentration in plasma samples, giving an intra-assay coefficient of variation of about 5% and an interassay coefficient of variation of about 9%. The sensitivity of the assay was found to be as low as 0.1 ng/ml of bPRL.
Prolactin, bovine; Elisa; biotin-avidin; plasma, bovine
Settore BIO/12 - Biochimica Clinica e Biologia Molecolare Clinica
Settore VET/02 - Fisiologia Veterinaria
1991
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/513165
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