The capacity to control immune responses and oxidative stresses of diet components is well known (1). Legumes and pseudo-cereals are source of proteins and peptides whose role in human body homeostasis mechanisms is still under-explored (2,3,4,5). Aim of this work was to identify protein or peptide fractions from lupin, quinoa, amaranth and buckwheat seeds, endowed with radical-scavenging activity and able to reduce inflammation in an intestinal cell model. Albumin and globulins were differentially solubilized from seed flours. Globulins were further separated in three fractions named 2S, 7S and 11S. Peptides were obtained by gastro-intestinal simulated digestion of all the mentioned protein families. The anti-oxidant activity was assessed by a DPPH-based method. Immune-modulation activity was tested by measuring the activation of NFkB pathway in cytokine-stimulated Caco-2 cells, transfected with the plasmid pNiFty2-Luc. Buckwheat proteins and peptides showed the maximum antioxidant activity. In amaranth, both integer and digested albumin had anti-oxidant activity, whereas globulins were effective only after digestion. Quinoa albumin, 2S globulin and digested 7S globulin were active. In lupin, albumin and digested 2S and 7S globulin resulted effective. The inflammatory response decreased of 70-80% in Caco-2 cells incubated with albumins of pseudo-cereals; of 60-30% with 2S globulins and about 30% with 7S and 11S globulins. Lupin proteins did not show positive immune-modulation effects. The immuno-modulation and anti-oxidative activities of specific protein fractions from pseudo- cereal and lupin seeds has been unveiled. This study opens the way to further studies addressed to the identification of the single active polypeptides with the aim to implement fortified and innovative foods. 1.Sandrè et al (2001) J Nutr 131, 2936-42 2.Duranti (2006) Fitoterapia 77, 67-82 3.Aluko e Monu, (2003) J Food Sci 68, 1254–58 4.Silva-Sánchez et al (2008) J Agric Food Chem 56, 1233-40 5.Metzger et al (2007) J Agric Food Chem 55, 6032-38

Anti-oxidant and immune-modulation activities of proteins and peptides from lupin, quinoa, amaranth and buckwheat seeds / J. Capraro, V. Artusa, S. Marulo, A. Scarafoni. ((Intervento presentato al convegno A molecular view of the food-health relationship tenutosi a Spetses nel 2017.

Anti-oxidant and immune-modulation activities of proteins and peptides from lupin, quinoa, amaranth and buckwheat seeds

J. Capraro;A. Scarafoni
2017

Abstract

The capacity to control immune responses and oxidative stresses of diet components is well known (1). Legumes and pseudo-cereals are source of proteins and peptides whose role in human body homeostasis mechanisms is still under-explored (2,3,4,5). Aim of this work was to identify protein or peptide fractions from lupin, quinoa, amaranth and buckwheat seeds, endowed with radical-scavenging activity and able to reduce inflammation in an intestinal cell model. Albumin and globulins were differentially solubilized from seed flours. Globulins were further separated in three fractions named 2S, 7S and 11S. Peptides were obtained by gastro-intestinal simulated digestion of all the mentioned protein families. The anti-oxidant activity was assessed by a DPPH-based method. Immune-modulation activity was tested by measuring the activation of NFkB pathway in cytokine-stimulated Caco-2 cells, transfected with the plasmid pNiFty2-Luc. Buckwheat proteins and peptides showed the maximum antioxidant activity. In amaranth, both integer and digested albumin had anti-oxidant activity, whereas globulins were effective only after digestion. Quinoa albumin, 2S globulin and digested 7S globulin were active. In lupin, albumin and digested 2S and 7S globulin resulted effective. The inflammatory response decreased of 70-80% in Caco-2 cells incubated with albumins of pseudo-cereals; of 60-30% with 2S globulins and about 30% with 7S and 11S globulins. Lupin proteins did not show positive immune-modulation effects. The immuno-modulation and anti-oxidative activities of specific protein fractions from pseudo- cereal and lupin seeds has been unveiled. This study opens the way to further studies addressed to the identification of the single active polypeptides with the aim to implement fortified and innovative foods. 1.Sandrè et al (2001) J Nutr 131, 2936-42 2.Duranti (2006) Fitoterapia 77, 67-82 3.Aluko e Monu, (2003) J Food Sci 68, 1254–58 4.Silva-Sánchez et al (2008) J Agric Food Chem 56, 1233-40 5.Metzger et al (2007) J Agric Food Chem 55, 6032-38
mag-2017
Settore BIO/10 - Biochimica
Anti-oxidant and immune-modulation activities of proteins and peptides from lupin, quinoa, amaranth and buckwheat seeds / J. Capraro, V. Artusa, S. Marulo, A. Scarafoni. ((Intervento presentato al convegno A molecular view of the food-health relationship tenutosi a Spetses nel 2017.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/499368
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