We have recently found that microbial species ranging from Gramnegative and Gram-positive bacteria to different fungi emit volatiles that strongly promote starch accumulation in leaves of both mono- and di-cotyledonous plants. Transcriptome and enzyme activity analyses of potato leaves exposed to volatiles emitted by Alternaria alternate revealed that starch over-accumulation was accompanied by enhanced 3-phosphoglycerate to Pi ratio, and changes in functions involved in both central carbohydrate and amino acid metabolism. Exposure to microbial volatiles also promoted changes in the expression of genes that code for enzymes involved in endocytic uptake and traffic of solutes. With the overall data we propose a metabolic model wherein important determinants of accumulation of exceptionally high levels of starch include (a) upregulation of ADPglucose-producing SuSy, starch synthase III and IV, proteins involved in the endocytic uptake and traffic of sucrose, (b) downregulation of acid invertase, starch breakdown enzymes and proteins involved in internal amino acid provision and (c) 3-phosphoglycerate-mediated allosteric activation of ADPglucose pyrophosphorylase.

A suggested model for potato MIVOISAP involving functions of central carbohydrate and amino acid metabolism, as well as actin cytoskeleton and endocytosis / I. Ezquer, J. Li, M. Ovecka, E. Baroja Fern�ez, F.J.M.M. Montero, J.D. de Cerio, M. Hidalgo, M.T. Sesma, A. Bahaji, E. Etxeberria, J. Pozueta Romero. - In: PLANT SIGNALING & BEHAVIOR. - ISSN 1559-2316. - 5:12(2010 Dec), pp. -5.

A suggested model for potato MIVOISAP involving functions of central carbohydrate and amino acid metabolism, as well as actin cytoskeleton and endocytosis

I. Ezquer
Primo
;
2010

Abstract

We have recently found that microbial species ranging from Gramnegative and Gram-positive bacteria to different fungi emit volatiles that strongly promote starch accumulation in leaves of both mono- and di-cotyledonous plants. Transcriptome and enzyme activity analyses of potato leaves exposed to volatiles emitted by Alternaria alternate revealed that starch over-accumulation was accompanied by enhanced 3-phosphoglycerate to Pi ratio, and changes in functions involved in both central carbohydrate and amino acid metabolism. Exposure to microbial volatiles also promoted changes in the expression of genes that code for enzymes involved in endocytic uptake and traffic of solutes. With the overall data we propose a metabolic model wherein important determinants of accumulation of exceptionally high levels of starch include (a) upregulation of ADPglucose-producing SuSy, starch synthase III and IV, proteins involved in the endocytic uptake and traffic of sucrose, (b) downregulation of acid invertase, starch breakdown enzymes and proteins involved in internal amino acid provision and (c) 3-phosphoglycerate-mediated allosteric activation of ADPglucose pyrophosphorylase.
ADPglucose; carbohydrate metabolism; microbial volatiles; starch; sucrose synthase; plant science; medicine (all)
Settore BIO/01 - Botanica Generale
Settore BIO/04 - Fisiologia Vegetale
dic-2010
http://www.landesbioscience.com/journals/psb/EzquerPSB5-12.pdf
Article (author)
File in questo prodotto:
File Dimensione Formato  
Ezquer_PlantSignalising_SuggestedModel_2010.pdf

accesso riservato

Tipologia: Publisher's version/PDF
Dimensione 499.35 kB
Formato Adobe PDF
499.35 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/488841
Citazioni
  • ???jsp.display-item.citation.pmc??? 2
  • Scopus 6
  • ???jsp.display-item.citation.isi??? ND
social impact