A strategy is described that enables the in situ detection of natural transformation in Acinetobacter baylyi BD413 by the expression of a green fluorescent protein. Microscale detection of bacterial transformants growing on plant tissues was shown by fluorescence microscopy and indicated that cultivation-based selection of transformants on antibiotic-containing agar plates underestimates transformation frequencies. Copyright
Strategy for in situ detection of natural transformation-based horizontal gene transfer events / A. Rizzi, A. Pontiroli, L. Brusetti, S. Borin, C. Sorlini, A. Abruzzese, G.A. Sacchi, T.M. Vogel, P. Simonet, M. Bazzicalupo, K.M. Nielsen, J.M. Monier, D. Daffonchio. - In: APPLIED AND ENVIRONMENTAL MICROBIOLOGY. - ISSN 0099-2240. - 74:4(2008), pp. 1250-1254.
Strategy for in situ detection of natural transformation-based horizontal gene transfer events
A. Rizzi;L. Brusetti;S. Borin;C. Sorlini;A. Abruzzese;G.A. Sacchi;D. Daffonchio
2008
Abstract
A strategy is described that enables the in situ detection of natural transformation in Acinetobacter baylyi BD413 by the expression of a green fluorescent protein. Microscale detection of bacterial transformants growing on plant tissues was shown by fluorescence microscopy and indicated that cultivation-based selection of transformants on antibiotic-containing agar plates underestimates transformation frequencies. Copyright
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