Filodinamica, filogeografia e caratterizzazione molecolare = Full genome di bovine viral diarrhoea virus (BVDV)

BVDV-1 is a Pestivirus with large impact on cattle worldwide. 5’-UTR sequences of the three main Italian BVDV-1 subtypes were considered for phylogeography. High similarity clusters were identified, and the relative sequences used to simulate transmission chains. No significant geographic structure was observed in BVDV-1b tree; BVDV-1e tree branched into three main clades, with Piedmontese sequences clustered together, either due to later sample collection or to a recent introduction of BVDV-1e. BVDV-1f sequences clustered into three significant clades: all Piedmont sequences clustered in two subclades, one including Aosta Valley sequences. Viruses with identical 5´-UTRs, suggesting transmission between farms, were identified, and their Npro sequences were used to select clusters for further epidemiological investigations. Finally, full genome sequences were generated by massive parallel sequencing (MPS) to study viral quasispecies from mucosal disease cases.