DNA interstrand cross-links (ICLs) are highly cytotoxic DNA lesions hindering DNA replication and transcription. Whereas in bacteria and yeast the molecular mechanisms involved in ICL repair are genetically well dissected, the scenario in multicellular organisms remains unclear. Here, we report that the two new mus308 genes, polq-1 and hel-308 are involved in ICL repair in Caenorhabditis elegans. After treatment with ICL agents, a decrease in survival and an increase in checkpoint-induced cell cycle arrest and apoptosis of germ cells is observed in mutants of both genes. Although sensitive to ICL agents and to a minor extent to IR radiation, cytological and epistatic analyses suggest that polq-1 and hel-308 are involved in different DNA repair pathways. While hel-308 functions in a Fanconi anemia dependent pathway, polq-1 has a role in a novel distinct and brc-1 (CeBRCA1)-dependent ICL repair process in metazoans.

Caenorhabditis elegans POLQ-1 and HEL-308 function in two distinct DNA interstrand cross-link repair pathways / D.M. Muzzini, P. Plevani, S.J. Boulton, G. Cassata, F. Marini. - In: DNA REPAIR. - ISSN 1568-7864. - 7:6(2008 Jun 01), pp. 941-950.

Caenorhabditis elegans POLQ-1 and HEL-308 function in two distinct DNA interstrand cross-link repair pathways

D.M. Muzzini
Primo
;
P. Plevani
Secondo
;
F. Marini
Ultimo
2008

Abstract

DNA interstrand cross-links (ICLs) are highly cytotoxic DNA lesions hindering DNA replication and transcription. Whereas in bacteria and yeast the molecular mechanisms involved in ICL repair are genetically well dissected, the scenario in multicellular organisms remains unclear. Here, we report that the two new mus308 genes, polq-1 and hel-308 are involved in ICL repair in Caenorhabditis elegans. After treatment with ICL agents, a decrease in survival and an increase in checkpoint-induced cell cycle arrest and apoptosis of germ cells is observed in mutants of both genes. Although sensitive to ICL agents and to a minor extent to IR radiation, cytological and epistatic analyses suggest that polq-1 and hel-308 are involved in different DNA repair pathways. While hel-308 functions in a Fanconi anemia dependent pathway, polq-1 has a role in a novel distinct and brc-1 (CeBRCA1)-dependent ICL repair process in metazoans.
English
Caenorhabditis elegans; Checkpoints; Fanconi anemia; ICL repair; RAD-51
Settore BIO/11 - Biologia Molecolare
Articolo
Esperti anonimi
Ricerca di base
Pubblicazione scientifica
1-giu-2008
Elsevier Science
7
6
941
950
10
Pubblicato
Periodico con rilevanza internazionale
info:eu-repo/semantics/article
Caenorhabditis elegans POLQ-1 and HEL-308 function in two distinct DNA interstrand cross-link repair pathways / D.M. Muzzini, P. Plevani, S.J. Boulton, G. Cassata, F. Marini. - In: DNA REPAIR. - ISSN 1568-7864. - 7:6(2008 Jun 01), pp. 941-950.
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Prodotti della ricerca::01 - Articolo su periodico
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262
Article (author)
si
D.M. Muzzini, P. Plevani, S.J. Boulton, G. Cassata, F. Marini
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/42082
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