Tissue healing is a complex process involving several players such as cells and growth factors released from platelets upon activation. Today, platelet concentrates (PCs) are used in many different medical fields including oral, orthopaedic, and reconstructive surgery since they allow growth factors delivery to the injured site, aiming at enhancing tissue regeneration. The purpose of this in vitro study was to evaluate the effect of the acellular plasma of an activated platelet concentrate obtained using a manual protocol, on the proliferation, and biological activity of differentiated cells involved in tissue healing. Human osteoblasts and dermal fibroblasts were grown in serum-free medium supplemented with PC derived from several donors. Human osteoblast and human dermal fibroblast proliferation was assessed by MTT test after 7 days and cells were count up to 12-day incubation. Human osteoblast osteo-differentiation was tested after 7 and 14-day incubation by alkaline phosphatase assay. The addition of PC to the culture medium caused an increased proliferation with respect to cells grown in standard condition. The results of the present study suggest that PC supports the proliferation of terminally differentiated cells involved in wound healing and tissue regeneration, confirming its beneficial clinical application in regenerative therapies.

Effect of an activated platelet concentrate on differentiated cells involved in tissue healing / A.T. Brini, C. Ceci, S. Taschieri, S. Niada, A. Lolato, C. Giannasi, C. Mortellaro, M. Del Fabbro. - In: THE JOURNAL OF CRANIOFACIAL SURGERY. - ISSN 1049-2275. - 27:3(2016 May), pp. 656-661.

Effect of an activated platelet concentrate on differentiated cells involved in tissue healing

A.T. Brini
Primo
;
C. Ceci
Secondo
;
S. Taschieri;S. Niada;A. Lolato;C. Giannasi;M. Del Fabbro
Ultimo
2016

Abstract

Tissue healing is a complex process involving several players such as cells and growth factors released from platelets upon activation. Today, platelet concentrates (PCs) are used in many different medical fields including oral, orthopaedic, and reconstructive surgery since they allow growth factors delivery to the injured site, aiming at enhancing tissue regeneration. The purpose of this in vitro study was to evaluate the effect of the acellular plasma of an activated platelet concentrate obtained using a manual protocol, on the proliferation, and biological activity of differentiated cells involved in tissue healing. Human osteoblasts and dermal fibroblasts were grown in serum-free medium supplemented with PC derived from several donors. Human osteoblast and human dermal fibroblast proliferation was assessed by MTT test after 7 days and cells were count up to 12-day incubation. Human osteoblast osteo-differentiation was tested after 7 and 14-day incubation by alkaline phosphatase assay. The addition of PC to the culture medium caused an increased proliferation with respect to cells grown in standard condition. The results of the present study suggest that PC supports the proliferation of terminally differentiated cells involved in wound healing and tissue regeneration, confirming its beneficial clinical application in regenerative therapies.
fibroblasts; growth factors; osteoblasts; opsteodifferentiation; proliferation; otorhinolaryngology; pathology and forensic medicine; surgery
Settore MED/28 - Malattie Odontostomatologiche
Settore BIO/14 - Farmacologia
Settore BIO/13 - Biologia Applicata
mag-2016
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/417957
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