Chemical composition and volatile constituents of Anthyllis barba-jovis

Phytochemical investigation of chloroformic and methanolic extracts from aerial parts of Anthyllis barba-jovis L. (Leguminosae) led to the characterization of six flavonol-glycosides: kaempferol-3-O--D-glucopyranoside, kaempferol-7-O--L-rhamnopyranoside, kaempferol-3-O--D-glucopyranoside-7-O--L-rhamnopyranoside, kaempferol-3-O--D-glucopyranosyl-(12)--L-rhamnopyranoside-7-O--L-rhamnopyranoside, quercetin-3-O--D-glucopyranoside, isorhamnetin-3-O--D-glucopyranoside-7-O--L-rhamnopyranoside were identified together with two coumarins and D-pinitol, a taxonomic marker of Leguminosae family. The structural elucidation of all compounds was based on their 1H and 13C-NMR spectral data, bidimensional experiments and confirmed by MS analysis. In order to complete the phytochemical investigation, the essential oils from flowers and seeds of A. barba-jovis were obtained by hydrodistillation and analyzed by GC/MS; fresh flowers were submitted also to Solid Phase Micro Extraction.