Development of a capillary electrophoresis method for urinary lactulose and mannitol determination to assess the intestinal permeability in patients with type 1 diabetes. The assessment of the unbalanced intestinal permeability to lactulose (LAC) and mannitol (MAN) after oral administration is used for the diagnosis of gluten-sensitive enteropathy. Aim of this study was to set up a method by capillary electrophoresis to detect the concentration of MAN and LAC in urine to estimate the intestinal permeability in controls and in patients affected by type I diabetes mellitus. In <10 min the underivatized carbohydrates were separated at pH of 12.5 and with indirect UV detection at 254 nm. In controls (n=41) 11.3 (median; interquartile range: 5.7-15.3) and 303 (237-440) mg of LAC and MAN were excreted during the 5 h urine collection after the carbohydrate administration, while in diabetes patients (n=24) 16.5 (12.8-24.3) and 229 (170-354) mg of LAC and MAN were found. This simple and rapid technique is suitable to study intestinal permeability alterations in different cohorts of patients
Sviluppo di un metodo in elettroforesi capillare per la determinazione del lattulosio e del mannitolo nelle urine e sue applicazioni allo studio della permeabilità intestinale in diabetici di tipo 1 / R. Paroni, I. Fermo, L. Molteni, L. Folini, M.R. Pastore, A. Mosca, E. Bosi. - In: BIOCHIMICA CLINICA. - ISSN 0393-0564. - 31:2(2007), pp. 118-121.
Sviluppo di un metodo in elettroforesi capillare per la determinazione del lattulosio e del mannitolo nelle urine e sue applicazioni allo studio della permeabilità intestinale in diabetici di tipo 1
R. ParoniPrimo
;A. MoscaPenultimo
;
2007
Abstract
Development of a capillary electrophoresis method for urinary lactulose and mannitol determination to assess the intestinal permeability in patients with type 1 diabetes. The assessment of the unbalanced intestinal permeability to lactulose (LAC) and mannitol (MAN) after oral administration is used for the diagnosis of gluten-sensitive enteropathy. Aim of this study was to set up a method by capillary electrophoresis to detect the concentration of MAN and LAC in urine to estimate the intestinal permeability in controls and in patients affected by type I diabetes mellitus. In <10 min the underivatized carbohydrates were separated at pH of 12.5 and with indirect UV detection at 254 nm. In controls (n=41) 11.3 (median; interquartile range: 5.7-15.3) and 303 (237-440) mg of LAC and MAN were excreted during the 5 h urine collection after the carbohydrate administration, while in diabetes patients (n=24) 16.5 (12.8-24.3) and 229 (170-354) mg of LAC and MAN were found. This simple and rapid technique is suitable to study intestinal permeability alterations in different cohorts of patientsPubblicazioni consigliate
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