Alterations of arginine plasma levels are involved in several disorders of amino acid metabolism such as hurtnup, argininosuccinic aciduria, histidinemia, citrullinuria, and cystinuria. In this work a new liquid ionization source, surface-activated chemical ionization (SACI), has been used to analyze arginine in human and rat plasma samples. Arginine was extracted and diluted ten times through protein precipitation. The diluted arginine samples were then analyzed using an ionexchange chromatographic column coupled with the SACI source and an ion trap analyzer using MS3 monitoring in order to increase the sensitivity and specificity of the analysis. The multiplepoint standard additions method was used to quantify the arginine. This method was employed to eliminate the matrix effect that affects all liquid ionization sources (APCI, ESI, SACI, etc.), and also does not require use of an internal standard. High-quality results in terms of sensitivity, limit of detection, lower limit of quantitation, linearity and reproducibility, are demonstrated.
|Titolo:||Surface-activated chemical ionization in the analysis of arginine in plasma samples|
|Parole Chiave:||Surface ; Active Agents ; Arginine|
|Settore Scientifico Disciplinare:||Settore BIO/12 - Biochimica Clinica e Biologia Molecolare Clinica|
Settore BIO/10 - Biochimica
|Data di pubblicazione:||2005|
|Digital Object Identifier (DOI):||10.1002/rcm.1921|
|Appare nelle tipologie:||01 - Articolo su periodico|