Rice (Oryza sativa) is the staple food of about half the world‟s human population. Enhancement of the rice grain‟s nutritional value through genetic improvement could include both increasing concentration of desirable elements (e.g. Fe, Zn, Ca, Mg or Cu) and decreasing concentrations of undesirables elements (e.g. As, Cd or Ni). Knowing the genetic basis of the ion content in rice grains is essential for understanding the mechanisms of nutrient transport and accumulation. This work aims at revealing how soil water management affects the rice ionome and identifying genes putatively involved in the micronutrients accumulation or in the exclusion of toxic trace elements in rice grain. To this purpose, a germplasm collection of 295 temperate rice genotypes was grown under flooded or unflooded conditions at CRA-RIS (Vercelli). Grain samples were analyzed by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) technique, in order to measure the concentration of 13 elements, including essential nutrients and toxic trace elements. The obtained ionomic maps show that in the majority of the cultivars the content of Cu, Ni and Cd was higher in plants grown under unflooded condition, whereas the content of As was higher in plants grown under flooded condition. Interestingly, a large variability in the grain concentrations of micronutrients and of trace elements was observed among the 295 cultivars grown under both unflooded and flooded conditions. The germplasm collection has been previously genotyped using a Genotyping by Sequencing approach, with a coverage of about 57.000 SNPs. A Genome Wide Association Study (GWAS) was applied to the ionomic data to identify loci putatively involved in nutrient transport and accumulation. A preliminary analysis on data regarding the first year of harvesting highlighted the presence of significant signals in correspondence of, or very close to, loci encoding for metal transporters (ATP-Binding Cassette or Natural Resist-Associate Macrophage Protein - Nramp) or for No Apical Meristem protein involved in the remobilization of nutrients from leaves to grains. Other positive signals were found in correspondence of loci codifying for phenylalanine ammonia-lyase, metallothioneins and several oxidoreductase activities. The GWAS of the second year of harvesting is still in progress.

Genome-wide dissection of rice grain ionome and its relationships to field water managment / M. Porrini, E. Baldoni, G. Lucchini, G. Orasen, G.V.F.F. Nocito, R. Greco, P. Abbruscato, P. Piffanelli, G.A. Sacchi. ((Intervento presentato al 33. convegno Convegno nazionale della Società Italiana di Chimica Agraria tenutosi a Bologna nel 2015.

Genome-wide dissection of rice grain ionome and its relationships to field water managment

M. Porrini
Primo
;
E. Baldoni
Secondo
;
G. Lucchini;G. Orasen;G.V.F.F. Nocito;G.A. Sacchi
Ultimo
2015

Abstract

Rice (Oryza sativa) is the staple food of about half the world‟s human population. Enhancement of the rice grain‟s nutritional value through genetic improvement could include both increasing concentration of desirable elements (e.g. Fe, Zn, Ca, Mg or Cu) and decreasing concentrations of undesirables elements (e.g. As, Cd or Ni). Knowing the genetic basis of the ion content in rice grains is essential for understanding the mechanisms of nutrient transport and accumulation. This work aims at revealing how soil water management affects the rice ionome and identifying genes putatively involved in the micronutrients accumulation or in the exclusion of toxic trace elements in rice grain. To this purpose, a germplasm collection of 295 temperate rice genotypes was grown under flooded or unflooded conditions at CRA-RIS (Vercelli). Grain samples were analyzed by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) technique, in order to measure the concentration of 13 elements, including essential nutrients and toxic trace elements. The obtained ionomic maps show that in the majority of the cultivars the content of Cu, Ni and Cd was higher in plants grown under unflooded condition, whereas the content of As was higher in plants grown under flooded condition. Interestingly, a large variability in the grain concentrations of micronutrients and of trace elements was observed among the 295 cultivars grown under both unflooded and flooded conditions. The germplasm collection has been previously genotyped using a Genotyping by Sequencing approach, with a coverage of about 57.000 SNPs. A Genome Wide Association Study (GWAS) was applied to the ionomic data to identify loci putatively involved in nutrient transport and accumulation. A preliminary analysis on data regarding the first year of harvesting highlighted the presence of significant signals in correspondence of, or very close to, loci encoding for metal transporters (ATP-Binding Cassette or Natural Resist-Associate Macrophage Protein - Nramp) or for No Apical Meristem protein involved in the remobilization of nutrients from leaves to grains. Other positive signals were found in correspondence of loci codifying for phenylalanine ammonia-lyase, metallothioneins and several oxidoreductase activities. The GWAS of the second year of harvesting is still in progress.
set-2015
Settore AGR/13 - Chimica Agraria
Genome-wide dissection of rice grain ionome and its relationships to field water managment / M. Porrini, E. Baldoni, G. Lucchini, G. Orasen, G.V.F.F. Nocito, R. Greco, P. Abbruscato, P. Piffanelli, G.A. Sacchi. ((Intervento presentato al 33. convegno Convegno nazionale della Società Italiana di Chimica Agraria tenutosi a Bologna nel 2015.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/318499
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