Many different methodologies have been applied for the detection of S-nitrosothiols (RSNOs) in human biological fluids. One unsatisfactory outcome of the last 14 years of research focused on this issue is that a general consensus on reference values for physiological RSNO concentration in human blood is still missing. Consequently, both RSNO physiological function and their role in disease have not yet been clarified. Here, a summary of the values measured for RSNOs in erythrocytes, plasma, and other biological fluids is provided, together with a critical review of the most widely used analytical methods. Furthermore, some possible methodological drawbacks, responsible for the highlighted discrepancies, are evidenced.

Detection of S-nitrosothiols in biological fluids : a comparison among the most widely applied methodologies / D. Giustarini, A. Milzani, I. Dalle Donne, R. Rossi. - In: JOURNAL OF CHROMATOGRAPHY. B. - ISSN 1570-0232. - 851:1-2(2007), pp. 124-139. [10.1016/j.jchromb.2006.09.031]

Detection of S-nitrosothiols in biological fluids : a comparison among the most widely applied methodologies

A. Milzani;I. Dalle Donne;
2007

Abstract

Many different methodologies have been applied for the detection of S-nitrosothiols (RSNOs) in human biological fluids. One unsatisfactory outcome of the last 14 years of research focused on this issue is that a general consensus on reference values for physiological RSNO concentration in human blood is still missing. Consequently, both RSNO physiological function and their role in disease have not yet been clarified. Here, a summary of the values measured for RSNOs in erythrocytes, plasma, and other biological fluids is provided, together with a critical review of the most widely used analytical methods. Furthermore, some possible methodological drawbacks, responsible for the highlighted discrepancies, are evidenced.
reviews; nitric oxide; S-nitrosoalbumin; S-nitrosohemoglobin; S-transnitrosation reactions
Settore BIO/06 - Anatomia Comparata e Citologia
2007
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/41883
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