Crown rot is a postharvest fungal disease with a great negative impact on fruit quality. It is caused by several fungal pathogens, including some Fusarium species. We studied Fusarium species associated to crown rot of organic bananas grown in Dominican Republic. Bananas were collected in five organic farms and in the corresponding packing stations over two years. A total of 316 hands were collected and more than 2034 fungal colonies were obtained from crown tissues. Out of them 275 representative Fusarium colonies were purified, characterized and identified. Combinations of two identification keys were used for morphological identification. In addition to, these isolates were characterized by DNA sequencing of nuclear rDNA internal transcribed spacer (ITS), beta-tubulin gene and microsatellite markers. Finally, 11 representative strains were tested for their pathogenicity. Fusarium spp. were isolated from all the analyzed samples from field and packing stations. Moreover they were isolated from different crown layer and also from crown internal tissues. The Fusarium population was composed mainly by seven species and five were the most frequent: F. verticillioides, F. chlamydosporum, F. solani, F. equiseti, and F. oxysporum. F. verticillioides was the most virulent specie which caused the highest disease incidence and severity in experimental pathogenicity tests.

Morphological and molecular characterization of Fusarium spp. isolated from Crown rot of organic bananas / M.A.M. Kamel, M. Saracchi, P. Cortesi - In: International Plant Protection Congress: Mission possible: food for all through appropriate plant protection : book of Abstracts[s.l] : German Scientific Society for Plant Protection Sciences., 2015 Aug. - ISBN 9783981650877. - pp. 441-441 (( Intervento presentato al 18. convegno International Plant Protection Congress tenutosi a Berlin nel 2015.

Morphological and molecular characterization of Fusarium spp. isolated from Crown rot of organic bananas

M.A.M. Kamel
Primo
;
M. Saracchi
Secondo
;
P. Cortesi
Ultimo
2015

Abstract

Crown rot is a postharvest fungal disease with a great negative impact on fruit quality. It is caused by several fungal pathogens, including some Fusarium species. We studied Fusarium species associated to crown rot of organic bananas grown in Dominican Republic. Bananas were collected in five organic farms and in the corresponding packing stations over two years. A total of 316 hands were collected and more than 2034 fungal colonies were obtained from crown tissues. Out of them 275 representative Fusarium colonies were purified, characterized and identified. Combinations of two identification keys were used for morphological identification. In addition to, these isolates were characterized by DNA sequencing of nuclear rDNA internal transcribed spacer (ITS), beta-tubulin gene and microsatellite markers. Finally, 11 representative strains were tested for their pathogenicity. Fusarium spp. were isolated from all the analyzed samples from field and packing stations. Moreover they were isolated from different crown layer and also from crown internal tissues. The Fusarium population was composed mainly by seven species and five were the most frequent: F. verticillioides, F. chlamydosporum, F. solani, F. equiseti, and F. oxysporum. F. verticillioides was the most virulent specie which caused the highest disease incidence and severity in experimental pathogenicity tests.
crown rot; organic bananas; Fusarium spp.; identification; characterization;
Settore AGR/12 - Patologia Vegetale
ago-2015
International Association for the Plant Protection Sciences
Deutsche Phytomedizinische Gesellschaft e.V.
Julius Kühn-Institut, Federal Research Centre for Cultivated Plants
Book Part (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/295861
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