Casein phosphopeptides (CPPs), derived by casein proteolysis, can bind calcium ions and keep them in solution. In vitro studies have demonstrated CPP-induced cell calcium uptake, depending on the formation of (CPP + calcium) complexes and on the degree of differentiation of the intestinal cells. With the present study, we address the persistence of the complexes and of the CPP-induced calcium uptake in intestinal like cells after the digestion process, thus examining their eligibility to serve as nutraceuticals. A calcium-preloaded CPP preparation of commercial origin (Ca-CPPs) was subjected to in vitro digestion. The evolution of the supramolecular structure of the Ca-CPP complexes was studied using laser-light and X-ray scattering. The bioactivity of the pre- and post-digestion Ca-CPPs was determined in differentiated Caco2 and HT-29 cells by video imaging experiments using Fura-2. We found that Ca-CPP aggregates keep a complex supramolecular organization upon digestion, despite getting smaller in size and increasing internal calcium dispersion. Concomitantly and most interestingly, digested Ca-CPPs clearly enhance the uptake of calcium ions, especially in Caco2 cells. In contrast, digestion depletes the ability of post-loaded decalcified-CPPs (Ca-dekCPPs), with a weaker internal structure, to induce calcium uptake. The enhanced bioactivity reached upon digestion strongly suggests a recognized role of Ca-CPPs, in the form used here, as nutraceuticals.
Calcium bio accessibility and uptake by human intestinal like cells following in vitro digestion of casein phosphopeptide-calcium aggregates / S. Perego, E. Del Favero, P. De Luca, F. Dal Piaz, A. Fiorilli, L. Cantu’, A. Ferraretto. - In: FOOD & FUNCTION. - ISSN 2042-6496. - 6:6(2015 Jun 10), pp. 1796-1807.
|Titolo:||Calcium bio accessibility and uptake by human intestinal like cells following in vitro digestion of casein phosphopeptide-calcium aggregates|
DEL FAVERO, ELENA (Secondo)
CANTU', LAURA FRANCA (Penultimo)
FERRARETTO, ANITA (Ultimo)
|Settore Scientifico Disciplinare:||Settore MED/49 - Scienze Tecniche Dietetiche Applicate|
Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin)
|Data di pubblicazione:||10-giu-2015|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1039/c4fo00672k|
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