High-performance liquid chromatography (HPLC) is still today the technique of choice for the analysis of natural dyes in artistic objects and historic textiles, particularly in association with photodiode-array detection (PDA). In the last two decades surface-enhanced Raman spectroscopy (SERS) gained also increasing importance for these investigations thanks to its sensitivity and limited requirements in terms of sample quantity. In favour of SERS its high specificity in molecular recognition typical of vibrational spectroscopy should be mentioned, whereas this non-separative technique is obviously disadvantaged in the analyses of mixed chromophores, as is the case of many natural dyes and also of tints obtained by the combined use of different colorants. An optimized experimental setup combining the two techniques, HPLC-PDA and SERS, is proposed in the present work, allowing on-line SERS detection of different dyeing compounds eluting from the HPLC column. Examples are presented concerning some of the colorants most widely used in history, such as morin and luteolin for yellow dyes, alizarin, purpurin, laccaic, kermesic and carminic acids for red ones and indigotin for blue tints.
HPLC-PDA-SERS for the identification of flavonoid, anthraquinone and indigoid historical dyes / C. Zaffino, G. Bedini, G. Mazzola, S. Bruni. ((Intervento presentato al convegno FIP Annual Symposium on Frontiers in Photonics Science and Technology tenutosi a Durham nel 2015.
HPLC-PDA-SERS for the identification of flavonoid, anthraquinone and indigoid historical dyes
C. ZaffinoPrimo
;S. BruniUltimo
2015
Abstract
High-performance liquid chromatography (HPLC) is still today the technique of choice for the analysis of natural dyes in artistic objects and historic textiles, particularly in association with photodiode-array detection (PDA). In the last two decades surface-enhanced Raman spectroscopy (SERS) gained also increasing importance for these investigations thanks to its sensitivity and limited requirements in terms of sample quantity. In favour of SERS its high specificity in molecular recognition typical of vibrational spectroscopy should be mentioned, whereas this non-separative technique is obviously disadvantaged in the analyses of mixed chromophores, as is the case of many natural dyes and also of tints obtained by the combined use of different colorants. An optimized experimental setup combining the two techniques, HPLC-PDA and SERS, is proposed in the present work, allowing on-line SERS detection of different dyeing compounds eluting from the HPLC column. Examples are presented concerning some of the colorants most widely used in history, such as morin and luteolin for yellow dyes, alizarin, purpurin, laccaic, kermesic and carminic acids for red ones and indigotin for blue tints.Pubblicazioni consigliate
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