Eradication of virus by sanctuary sites is a main goal in HIV management. The central nervous system (CNS) is a classic model of sanctuary where viral replication occurs despite a complete viral suppression in peripheral blood. In recent years, nanotechnologies have provided a great promise in the eradication of HIV from the CNS. We hereby demonstrate for the first time that the structurally complex antiretroviral drug enfuvirtide (Enf), which normally is unable to penetrate the cerebrospinal fluid, is allowed to cross the blood brain barrier (BBB) in mice by conjugation with a nanoconstruct. Iron oxide nanoparticles coated with an amphiphilic polymer increase Enf translocation across the BBB in both in vitro and in vivo models. The mechanism involves the uptake of nanoconjugated-Enf in the endothelial cells, the nanocomplex dissociation and the release of the peptide, which is eventually excreted by the cells in the brain parenchyma.
Nanoformulation of antiretroviral drugs enhances their penetration across the blood brain barrier in mice / L. Fiandra, M. Colombo, S. Mazzucchelli, M. Truffi, B. Santini, R. Allevi, M. Nebuloni, A. Capetti, G. Rizzardini, D. Prosperi, F. Corsi. - In: NANOMEDICINE. - ISSN 1549-9634. - 11:6(2015), pp. 1387-1397. [10.1016/j.nano.2015.03.009]
Nanoformulation of antiretroviral drugs enhances their penetration across the blood brain barrier in mice
L. FiandraPrimo
;S. Mazzucchelli;M. Truffi;R. Allevi;M. Nebuloni;F. Corsi
2015
Abstract
Eradication of virus by sanctuary sites is a main goal in HIV management. The central nervous system (CNS) is a classic model of sanctuary where viral replication occurs despite a complete viral suppression in peripheral blood. In recent years, nanotechnologies have provided a great promise in the eradication of HIV from the CNS. We hereby demonstrate for the first time that the structurally complex antiretroviral drug enfuvirtide (Enf), which normally is unable to penetrate the cerebrospinal fluid, is allowed to cross the blood brain barrier (BBB) in mice by conjugation with a nanoconstruct. Iron oxide nanoparticles coated with an amphiphilic polymer increase Enf translocation across the BBB in both in vitro and in vivo models. The mechanism involves the uptake of nanoconjugated-Enf in the endothelial cells, the nanocomplex dissociation and the release of the peptide, which is eventually excreted by the cells in the brain parenchyma.File | Dimensione | Formato | |
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