Intracellular, loosely bound Ca++ has been localized electron microscopically in freshly ejaculated boar spermatozoa by in situ precipitation with potassium antimonate. Ca++ was identified as the cation precipitated by testing the EGTA-sensitivity of the precipitates and by X-ray microprobe analysis. The data obtained revealed that the outer acrosomal membrane is the preferential site for Ca++ precipitation in the sperm head.
Ca++ localization in boar spermatozoa by the pyroantimonate technique and X-ray microanalysis / G. Berruti, E. Francgi, M. Camatini. - In: JOURNAL OF EXPERIMENTAL ZOOLOGY. - ISSN 0022-104X. - 237:2(1986 Feb), pp. 257-262.
Ca++ localization in boar spermatozoa by the pyroantimonate technique and X-ray microanalysis
G. BerrutiPrimo
;
1986
Abstract
Intracellular, loosely bound Ca++ has been localized electron microscopically in freshly ejaculated boar spermatozoa by in situ precipitation with potassium antimonate. Ca++ was identified as the cation precipitated by testing the EGTA-sensitivity of the precipitates and by X-ray microprobe analysis. The data obtained revealed that the outer acrosomal membrane is the preferential site for Ca++ precipitation in the sperm head.
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