Sulphur dioxide (SO2) proved to increase absorbance at 280 nm of grape skin and seed extracts containing it, diluted with ethanol-HCl to assess total flavonoids and anthocyanins in the same analysis. Additional absorbance at 280 nm was also observed in acetone:H2O extracts, if the acetone had not completely evaporated before the extracts were diluted with a solvent. Flavonoids were correctly quantified in the extracts when SO2 or acetone were removed by solid-phase extraction with a C18 RP as sorbent and methanol as eluting solvent.
Over-evaluation of total flavonoids in grape skin extracts containing sulphur dioxide / O. Corona, M. Squadrito, G. Vento, A. Tirelli, R. Di Stefano. - In: FOOD CHEMISTRY. - ISSN 0308-8146. - 172(2015), pp. 537-542. [10.1016/j.foodchem.2014.09.125]
Over-evaluation of total flavonoids in grape skin extracts containing sulphur dioxide
A. TirelliPenultimo
;
2015
Abstract
Sulphur dioxide (SO2) proved to increase absorbance at 280 nm of grape skin and seed extracts containing it, diluted with ethanol-HCl to assess total flavonoids and anthocyanins in the same analysis. Additional absorbance at 280 nm was also observed in acetone:H2O extracts, if the acetone had not completely evaporated before the extracts were diluted with a solvent. Flavonoids were correctly quantified in the extracts when SO2 or acetone were removed by solid-phase extraction with a C18 RP as sorbent and methanol as eluting solvent.File | Dimensione | Formato | |
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Corona-FC 172 (2015)-Over-evaluation of total flavonoids in grape skin extracts containing sulphur dioxide.pdf
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