A subpopulation of scleroderma dermal fibroblasts was identified by flow cytometric analysis. Between 15% and 25% of the cells within the scleroderma fibroblast lines had high levels of cytoplasmic granularity, as identified by side light scatter characteristics. Similar fibroblasts composed less than 3% of the cells within the normal fibroblast lines, although greater numbers could be induced through exposure to soluble factors derived from activated mononuclear cells. The granular subpopulation of fibroblasts produced 2-3 times as much procollagen as did the other fibroblasts. These data support the hypothesis that fibrosis in scleroderma may result in part from the activity of an inherently high procollagen-producing subset of normal fibroblasts that is expanded through exposure to immune cytokines.

In vitro identification of a subpopulation of fibroblasts that produces high levels of collagen in scleroderma patients / B.W. Needleman, J.V. Ordonez, D. Taramelli, W. Alms, K. Gayer, J. Choi. - In: ARTHRITIS AND RHEUMATISM. - ISSN 0004-3591. - 33:6(1990 Jun), pp. 842-852.

In vitro identification of a subpopulation of fibroblasts that produces high levels of collagen in scleroderma patients

D. Taramelli;
1990

Abstract

A subpopulation of scleroderma dermal fibroblasts was identified by flow cytometric analysis. Between 15% and 25% of the cells within the scleroderma fibroblast lines had high levels of cytoplasmic granularity, as identified by side light scatter characteristics. Similar fibroblasts composed less than 3% of the cells within the normal fibroblast lines, although greater numbers could be induced through exposure to soluble factors derived from activated mononuclear cells. The granular subpopulation of fibroblasts produced 2-3 times as much procollagen as did the other fibroblasts. These data support the hypothesis that fibrosis in scleroderma may result in part from the activity of an inherently high procollagen-producing subset of normal fibroblasts that is expanded through exposure to immune cytokines.
adult; biological factors; cell line; collagen; cytokines; fibroblasts; flow cytometry; humans; middle aged; monocytes; procollagen; protein biosynthesis; reference values; reproducibility of results; scleroderma, localized; skin
Settore MED/04 - Patologia Generale
giu-1990
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/245174
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