Electrical parameters of the abdominal skin of the pithed frog (Rana esculenta) can be measured by means of a couple of double coaxial electrodes /1,2,3/. One of the double electrodes is inserted into the ventral lymphatic sac of the frog, between skin and muscles, and the second is placed in front of this, on the outer surface of the skin. The inner electrode of the coaxial pair measures the skin potential difference (pd), while the outer delivers a countercurrent, for modifying the pd. By this method, pd, short circuit current and skin DC resistance have been determined as a function of temperature (5 to 40 °C) and of pH (4 to 9) on the same living animal. The behaviour of the substrate bears both qualitative and quantitative similarities with the isolated skin in the lower temperature range, but no transport maximum exists around 27 °C /4/. The pH dependence of electrical parameters is also quite different than in the isolated substrate /5/. 1. G. Torelli, F. Celentano, G. Cortili, G. Guella: Boll. Soc. It. Biol. Sper. 44, 501 (1967); 2. F. Celentano, G. Cortili, G. Guella, G. Torelli: Boll. Soc. lt. Biol. Sper. 44, 504 (1967); 3. M. Bianchi, G. Torelli, F. Celentano, G. Cortili: Boll. Soc. It. Biol. Sper. 45, 385 (1968); 4. G.A. Poster: Biochim. Biophys. Acta 211, 487 (1970); 5. E. Schoffeniels: Arch. Int. Physiol. Biochim. 53, 513 (1955).
Measurements of electrical parameters of frog skin "in situ" as a function of environmental parameters / F. Celentano, G. Torelli, G. Monticelli. ((Intervento presentato al 4. convegno International Biophysics Congress tenutosi a Moscow nel 1972.
Measurements of electrical parameters of frog skin "in situ" as a function of environmental parameters
G. MonticelliUltimo
1972
Abstract
Electrical parameters of the abdominal skin of the pithed frog (Rana esculenta) can be measured by means of a couple of double coaxial electrodes /1,2,3/. One of the double electrodes is inserted into the ventral lymphatic sac of the frog, between skin and muscles, and the second is placed in front of this, on the outer surface of the skin. The inner electrode of the coaxial pair measures the skin potential difference (pd), while the outer delivers a countercurrent, for modifying the pd. By this method, pd, short circuit current and skin DC resistance have been determined as a function of temperature (5 to 40 °C) and of pH (4 to 9) on the same living animal. The behaviour of the substrate bears both qualitative and quantitative similarities with the isolated skin in the lower temperature range, but no transport maximum exists around 27 °C /4/. The pH dependence of electrical parameters is also quite different than in the isolated substrate /5/. 1. G. Torelli, F. Celentano, G. Cortili, G. Guella: Boll. Soc. It. Biol. Sper. 44, 501 (1967); 2. F. Celentano, G. Cortili, G. Guella, G. Torelli: Boll. Soc. lt. Biol. Sper. 44, 504 (1967); 3. M. Bianchi, G. Torelli, F. Celentano, G. Cortili: Boll. Soc. It. Biol. Sper. 45, 385 (1968); 4. G.A. Poster: Biochim. Biophys. Acta 211, 487 (1970); 5. E. Schoffeniels: Arch. Int. Physiol. Biochim. 53, 513 (1955).Pubblicazioni consigliate
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