2D DIGE, two-dimensional difference gel electrophoresis, is a technology used to study the protein expression on two-dimensional gels. Protein samples are labeled with different color fluorescent dyes designed not to affect the relative migration of proteins during electrophoresis. Here, we describe the practical procedures necessary to perform a 2D DIGE experiment for a muscle tissue protein extract followed by CyDye DIGE fluors minimal labeling and the analysis of 2D DIGE gels for the assessment of quantitative differences.
2D DIGE analysis of protein extracts from muscle tissue / C. Gelfi, S. De Palma (METHODS IN MOLECULAR BIOLOGY). - In: Difference Gel Electrophoresis (DIGE) : methods and protocols / [a cura di] R. Cramer, R. Westermeier. - New York : Humana Press, 2012. - ISBN 9781617795725. - pp. 155-168 [10.1007/978-1-61779-573-2_11]
2D DIGE analysis of protein extracts from muscle tissue
C. Gelfi;S. De Palma
2012
Abstract
2D DIGE, two-dimensional difference gel electrophoresis, is a technology used to study the protein expression on two-dimensional gels. Protein samples are labeled with different color fluorescent dyes designed not to affect the relative migration of proteins during electrophoresis. Here, we describe the practical procedures necessary to perform a 2D DIGE experiment for a muscle tissue protein extract followed by CyDye DIGE fluors minimal labeling and the analysis of 2D DIGE gels for the assessment of quantitative differences.
Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
