Toxoplasmosis is one of the major diseases in goats, causing reproductive and economic losses (Dubey et al., 2011). The aim of this study is to investigate the correspondence in the antibodies presence in sera and milk and their variation during the lactation. Besides, a perspective aim is to evaluate the possibility for milk to be used as a valid tool in the diagnosis of toxoplasmosis in small ruminants. The study was carried out from March to July 2013 in a herd in Varese province (Northern Italy) that presented previous, not diagnosed problems in fertility. Blood and milk samples were fortnightly collected from each animal (30 females), for seven times. The validation for milk of a commercial ELISA kit (ID Screen® Toxoplasmosis Indirect Multi-Species, IDVET, Montpellier) was performed by comparing milk and serum samples from 48 goats previously tested for T. gondii by IFAT (Camargo, 1974). Serum samples were diluted at 1:10, following the manufacturer instruction, and milk samples at 1:2. Nineteen sera resulted positive and a correlation between antibodies in milk and sera was found (Pearson’s correlation: p=0.000), although the antibody titre was lower in milk than in sera in all animals tested. Four positive goats with low antibodies titre in sera had no antibodies in milk. The level of antibodies in milk is in general lower than that in serum (Elvander et al., 1995), and it may explain why the low-positive sera were negative in milk samples. Testing of milk samples is easier and less expensive than testing of blood samples (Schares et al., 2004), and can be considered a good diagnostic tool for toxoplasmosis at flock level.

Detection of Toxoplasma gondii antibodies in sera and milk of naturally infected goats / A.L. Gazzonis, S.A.G. Zanzani, G. Molineri, M. Villa, M.T. Manfredi. ((Intervento presentato al convegno Final CAPARA Conference & MC Meeting tenutosi a Berlin nel 2013.

Detection of Toxoplasma gondii antibodies in sera and milk of naturally infected goats

A.L. Gazzonis;S.A.G. Zanzani;M.T. Manfredi
2013-12

Abstract

Toxoplasmosis is one of the major diseases in goats, causing reproductive and economic losses (Dubey et al., 2011). The aim of this study is to investigate the correspondence in the antibodies presence in sera and milk and their variation during the lactation. Besides, a perspective aim is to evaluate the possibility for milk to be used as a valid tool in the diagnosis of toxoplasmosis in small ruminants. The study was carried out from March to July 2013 in a herd in Varese province (Northern Italy) that presented previous, not diagnosed problems in fertility. Blood and milk samples were fortnightly collected from each animal (30 females), for seven times. The validation for milk of a commercial ELISA kit (ID Screen® Toxoplasmosis Indirect Multi-Species, IDVET, Montpellier) was performed by comparing milk and serum samples from 48 goats previously tested for T. gondii by IFAT (Camargo, 1974). Serum samples were diluted at 1:10, following the manufacturer instruction, and milk samples at 1:2. Nineteen sera resulted positive and a correlation between antibodies in milk and sera was found (Pearson’s correlation: p=0.000), although the antibody titre was lower in milk than in sera in all animals tested. Four positive goats with low antibodies titre in sera had no antibodies in milk. The level of antibodies in milk is in general lower than that in serum (Elvander et al., 1995), and it may explain why the low-positive sera were negative in milk samples. Testing of milk samples is easier and less expensive than testing of blood samples (Schares et al., 2004), and can be considered a good diagnostic tool for toxoplasmosis at flock level.
Settore VET/06 - Parassitologia e Malattie Parassitarie degli Animali
COST Action FA0805
COST
European Cooperation in Science and Technology
Detection of Toxoplasma gondii antibodies in sera and milk of naturally infected goats / A.L. Gazzonis, S.A.G. Zanzani, G. Molineri, M. Villa, M.T. Manfredi. ((Intervento presentato al convegno Final CAPARA Conference & MC Meeting tenutosi a Berlin nel 2013.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/236474.1
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