Multiview proteomic analysis of Pseudomonas aeruginosa cell envelope district

We report on a multiview proteomic approach to the Pseudomonas aeruginosa cell envelope district that combined three “classical” methods for the analysis of membrane proteins, i.e. protease shaving on isolated membranes, membrane proteins extraction, surface shaving of intact cells, with a novel nanoparticle-based technique for the specific magneto-capture of surface exposed proteins [1]. This analysis identified more than 1600 unique peptides corresponding to 722 proteins. For 41% of these proteins, our analysis provided for the first time an experimental evidence of their actual expression, i.e. removed them from the status of either “hypothetical” or “probable” proteins. Moreover, this multiview analysis allowed a high-throughput membrane-related topological profiling of the envelope district proteome. 1. Vecchietti D, Di Silvestre D, Miriani M, Bonomi F, Marengo M, et al. (2012) Analysis of Pseudomonas aeruginosa cell envelope proteome by capture of surface-exposed proteins on activated magnetic nanoparticles. PLoS One 7: e51062.