Antibiofilm activity of selected natural compounds

To identify molecular targets that interact with zosteric acid, a small-scale affinity purification of proteins from E. coli extracts on immobilized zosteric acid moieties was performed. According to the biological screening of the small library of molecules, p-aminocinnamic acid was selected for its covalent coupling to the affinity support. Functionalization of the solid support was successfully demonstrated by fluorescence analysis. The protein pull-down revealed that one protein bound to cinnamate coupled matrix. The identification of this protein is still in progress.