Neuregulin1 is a family of growth and differentiation factors involved in various functions of both peripheral and central nervous system including the regenerative processes that underlie regeneration of damaged peripheral nerves. In the present study we tested in vitro the effect of Neuregulin1 administration on dissociated rat dorsal root ganglion (DRG). Activity of neuregulin1 was compared to the activity of nerve growth factor in the same in vitro experimental model. Results showed that neurite outgrowth is enhanced by the addition of both neuregulin1 and nerve growth factor to the culture medium. While neuregulin1 was responsible for the growth of longer neurites, DRG neurons incubated with nerve growth factor showed shorter and more branched axons. Using enzyme-linked immunosorbent assay we also showed that the release of nerve growth factor, but not of brain derived neurotrophic factor is improved in DRG neuron treated with neuregulin1. On the other hand, the assay with growth factor blocking antibody, showed that effects exerted by neuregulin1 on neurite outgrowth is only partially due to the release of nerve growth factor. Taken together the results of this study provide a better understanding on the role of neuregulin1 in sensory neurons.

Neuregulin1 administration increases axonal elongation in dissociated primary sensory neuron cultures / C. Audisio, C. Mantovani, S. Raimondo, S. Geuna, I. Perroteau, G. Terenghi. - In: EXPERIMENTAL CELL RESEARCH. - ISSN 0014-4827. - 318:5(2012 Mar 10), pp. 570-577.

Neuregulin1 administration increases axonal elongation in dissociated primary sensory neuron cultures

C. Mantovani
Secondo
;
2012

Abstract

Neuregulin1 is a family of growth and differentiation factors involved in various functions of both peripheral and central nervous system including the regenerative processes that underlie regeneration of damaged peripheral nerves. In the present study we tested in vitro the effect of Neuregulin1 administration on dissociated rat dorsal root ganglion (DRG). Activity of neuregulin1 was compared to the activity of nerve growth factor in the same in vitro experimental model. Results showed that neurite outgrowth is enhanced by the addition of both neuregulin1 and nerve growth factor to the culture medium. While neuregulin1 was responsible for the growth of longer neurites, DRG neurons incubated with nerve growth factor showed shorter and more branched axons. Using enzyme-linked immunosorbent assay we also showed that the release of nerve growth factor, but not of brain derived neurotrophic factor is improved in DRG neuron treated with neuregulin1. On the other hand, the assay with growth factor blocking antibody, showed that effects exerted by neuregulin1 on neurite outgrowth is only partially due to the release of nerve growth factor. Taken together the results of this study provide a better understanding on the role of neuregulin1 in sensory neurons.
Axons ; Animals ; Brain-Derived Neurotrophic Factor ; Cells, Cultured ; Ganglia, Spinal ; Male ; Nerve Growth Factor ; Neuregulin-1 ; Neurites ; Primary Cell Culture ; Rats ; Rats, Sprague-Dawley ; Sensory Receptor Cells ; Tubulin
Settore MED/13 - Endocrinologia
10-mar-2012
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/230999
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