We present here a new methodology for quantitative mapping of light elements in cells, based on combination of compositional and morphological information, derived respectively by X-ray Fluorescence Microscopy (XRFM), Atomic Force Microscopy and Scanning Transmission X-ray Microscopy (STXM). Since XRFM of light elements (carbon, nitrogen, oxygen, sodium and magnesium), are strongly influenced by self-absorption, we developed an algorithm to correct for this effect, using the morphological and structural information provided by AFM and STXM. Finally, the corrected distributions have been obtained, thus allowing quantitative mapping.

X-ray fluorescence microscopy of light elements in cells: self-absorption correction by integration of compositional and morphological measurements / E Malucelli , S. Iotti , M. Fratini , C. Marraccini , A. Notargiacomo , A. Gianoncelli , I. Bukreeva , A. Cedola , J. Maier , G. Farruggia , C. Cappadone , L. Merolle , F. Wolf , V. Trapani and S Lagomarsino. - In: JOURNAL OF PHYSICS. CONFERENCE SERIES. - ISSN 1742-6588. - 463:1(2013), p. 012022.012022. ((Intervento presentato al 11. convegno Conference on X-ray Microscopy - XRM2012 tenutosi a Shanghai nel 2013 [10.1088/1742-6596/463/1/012022].

X-ray fluorescence microscopy of light elements in cells: self-absorption correction by integration of compositional and morphological measurements

J. Maier;
2013

Abstract

We present here a new methodology for quantitative mapping of light elements in cells, based on combination of compositional and morphological information, derived respectively by X-ray Fluorescence Microscopy (XRFM), Atomic Force Microscopy and Scanning Transmission X-ray Microscopy (STXM). Since XRFM of light elements (carbon, nitrogen, oxygen, sodium and magnesium), are strongly influenced by self-absorption, we developed an algorithm to correct for this effect, using the morphological and structural information provided by AFM and STXM. Finally, the corrected distributions have been obtained, thus allowing quantitative mapping.
Settore MED/04 - Patologia Generale
2013
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/228739
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