AIM: To examine the efficacy on growth inhibition of 2-methoxyestradiol (2-ME) on human hepatocellular carcinoma in vitro. METHODS: Hep3B, SK-Hep1, and PLC/PRF/5 cells were used. Proliferation assays using 2-ME should show a dose-dependent reduction of cell number. Different staining methods in cells derived from human hepatocellular carcinoma and normal human hepatocytes were performed to demonstrate possible tumor specific induction of apoptosis. FACS-analysis was done to confirm the induction of apoptosis after treatment with 2-ME. RESULTS: A reduction of the cell number of 90-98% was observed in all cancer cells after treatment with 2 micromol 2-ME. The mechanism of action appeared to be induction of apoptosis. Normal human hepatocytes were unaffected by 2-ME. The most sensitive cell line to 2-ME, SK-Hep1, showed an up-regulation of the p53 and p21 proteins. CONCLUSIONS: 2-Methoxyestradiol appears to be highly effective in reducing tumor growth in vitro in human hepatocellular carcinoma. It may be tumor specific and applicable for clinical trials.
A very low toxic agent induces apoptosis and reduces growth of human hepatocellular carcinoma cells / G. Schumacher, S. Scheunert, A. Rueggeberg, M.G. Bachem, A.K. Nussler, A. Spinelli, T. Mukhopadhyay, J. Pratschke, P. Neuhaus. - In: JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY. - ISSN 0815-9319. - 21:7(2006 Jul), pp. 1207-1212. [10.1111/j.1440-1746.2006.04327.x]
A very low toxic agent induces apoptosis and reduces growth of human hepatocellular carcinoma cells
A. Spinelli;
2006
Abstract
AIM: To examine the efficacy on growth inhibition of 2-methoxyestradiol (2-ME) on human hepatocellular carcinoma in vitro. METHODS: Hep3B, SK-Hep1, and PLC/PRF/5 cells were used. Proliferation assays using 2-ME should show a dose-dependent reduction of cell number. Different staining methods in cells derived from human hepatocellular carcinoma and normal human hepatocytes were performed to demonstrate possible tumor specific induction of apoptosis. FACS-analysis was done to confirm the induction of apoptosis after treatment with 2-ME. RESULTS: A reduction of the cell number of 90-98% was observed in all cancer cells after treatment with 2 micromol 2-ME. The mechanism of action appeared to be induction of apoptosis. Normal human hepatocytes were unaffected by 2-ME. The most sensitive cell line to 2-ME, SK-Hep1, showed an up-regulation of the p53 and p21 proteins. CONCLUSIONS: 2-Methoxyestradiol appears to be highly effective in reducing tumor growth in vitro in human hepatocellular carcinoma. It may be tumor specific and applicable for clinical trials.
Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
