AIMS: a glutathione (GSH) yeast-based biomass (S. cerevisiae) was used to investigate GSH stability, solubilization during gastrointestinal digestion and GSH intestinal transport. METHODS AND RESULTS: a post-growing procedure was applied to improve intracellular GSH yeast content. The presence of adenine (ADE) in the biotransformation solution (CYS-GLY-GLU mixture) and alternatively, a glucose shot after 4 h incubation, allowed to obtain cells containing about GSH 1.6-1.7% dcw (dry cell weight) (control 0.5%). Yeast samples were subjected to in vitro gastro-intestinal digestion and absorption assays employing Caco-2 and HT29-MTX cell lines in different proportions (100/0, 70/30 and 50/50). Trials were also performed to verify intestinal cell viability. CONCLUSIONS: at least 87% of ingested GSH is available in reduced form for intestinal absorption. In vitro GSH transport assays indicated that GSH is poorly absorbed (<20%). Nevertheless, studies in response to oxidative stress induced by H2 O2 demonstrated a protective role of the GSH-enriched biomass towards intestinal cell viability. SIGNIFICANCE AND IMPACT OF STUDY: an enriched GSH yeast-based biomass has been obtained using a post-growing procedure. Although GSH present in enriched yeasts is poorly absorbed by intestinal cells, this biomass showed an intestinal local protective effect, improving cells viability when a simulated oxidative stress was applied.

Glutathione–enriched baker’s yeast: production, bioaccessibility and intestinal transport assays / A. Musatti, V. Devesa, M. Calatayud, D. Velez, M. Manzoni, M. Rollini. - 116:2(2014), pp. 304-313.

Glutathione–enriched baker’s yeast: production, bioaccessibility and intestinal transport assays

A. Musatti
Primo
;
M. Manzoni
Penultimo
;
M. Rollini
2014

Abstract

AIMS: a glutathione (GSH) yeast-based biomass (S. cerevisiae) was used to investigate GSH stability, solubilization during gastrointestinal digestion and GSH intestinal transport. METHODS AND RESULTS: a post-growing procedure was applied to improve intracellular GSH yeast content. The presence of adenine (ADE) in the biotransformation solution (CYS-GLY-GLU mixture) and alternatively, a glucose shot after 4 h incubation, allowed to obtain cells containing about GSH 1.6-1.7% dcw (dry cell weight) (control 0.5%). Yeast samples were subjected to in vitro gastro-intestinal digestion and absorption assays employing Caco-2 and HT29-MTX cell lines in different proportions (100/0, 70/30 and 50/50). Trials were also performed to verify intestinal cell viability. CONCLUSIONS: at least 87% of ingested GSH is available in reduced form for intestinal absorption. In vitro GSH transport assays indicated that GSH is poorly absorbed (<20%). Nevertheless, studies in response to oxidative stress induced by H2 O2 demonstrated a protective role of the GSH-enriched biomass towards intestinal cell viability. SIGNIFICANCE AND IMPACT OF STUDY: an enriched GSH yeast-based biomass has been obtained using a post-growing procedure. Although GSH present in enriched yeasts is poorly absorbed by intestinal cells, this biomass showed an intestinal local protective effect, improving cells viability when a simulated oxidative stress was applied.
Caco-2; Gastrointestinal digestion; Glutathione; HT29-MTX; Intestinal absorption; Yeast
Settore AGR/16 - Microbiologia Agraria
2014
Article (author)
File in questo prodotto:
File Dimensione Formato  
Musatti JAM revision 2.pdf

accesso aperto

Tipologia: Pre-print (manoscritto inviato all'editore)
Dimensione 387.58 kB
Formato Adobe PDF
387.58 kB Adobe PDF Visualizza/Apri
jam.12363.pdf

accesso aperto

Tipologia: Publisher's version/PDF
Dimensione 532.67 kB
Formato Adobe PDF
532.67 kB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/226490
Citazioni
  • ???jsp.display-item.citation.pmc??? 2
  • Scopus 12
  • ???jsp.display-item.citation.isi??? 10
social impact